Abstract.To simplify the task of building distributed streaming applications, we propose a new abstraction for information flow-Infopipes. Infopipes make information flow primary, not an auxiliary mechanism that is hidden away. Systems are built by connecting pre-defined component Infopipes such as sources, sinks, buffers, filters, broadcasting pipes, and multiplexing pipes. The goal of Infopipes is not to hide communication, like an RPC system, but to reify it: to represent communication explicitly as objects that the program can interrogate and manipulate. Moreover, these objects represent communication in application-level terms, not in terms of network or process implementation.
The kinetic, selective and stereoselective properties of enzyme immobilised on magnetic polymer beads with diameters in the range 1 microm was studied with penicillin amidase from E. coli. The enzyme was immobilised on epoxy and glutaraldehyde-activated poly(vinyl alcohol), poly(methylmetacrylate) and poly(vinyl acetate-divinylbenzene) magnetic beads. The amount of covalently bound active protein was dependent on the chemical modification of the matrix and increased at higher ionic strength of the immobilisation buffer. The small size of the magnetic beads, that reduces mass transfer limitations, and the decreased charge density in the electric double layer resulted in lower apparent Km values and higher efficiency for benzylpenicillin hydrolysis, higher stereoselectivity in condensation of R-phenylglycine amide with S- and R-Phe and in hydrolysis of racemic phenylacetyl-Phe and higher selectivity in kinetically controlled synthesis of cephalexin compared to the enzyme immobilised on larger and porous carriers.
A new type of Laser‐induced Breakdown Detection (LIBD) is being developed for the direct quantification of aquatic colloids of extremely small sizes at very low concentrations, which are not detectable by the commercial methods currently available. LIBD allows for the detection of colloids in the lower nanometer range. Different statistical and mathematical models are available for the quantification of colloids in aquatic systems. In this double publication (parts I and II), a model based on binomial statistics was employed. This is introduced as the basis of our theoretical descriptions of the experimental data discussed in part II. As a prerequisite, a qualitative and quantitative description of the power density distribution in the focal area of a laser beam is necessary and thus is also presented in this article. Together with the second part, this paper is intended to provide a profound insight into both the theoretical and experimental aspects of the Laser‐induced Breakdown Detection technique, and to show practical applications as well as the latest developments in this field of particle characterization.
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