Foodborne illnesses have become more common over time, posing a major threat to human health around the world. Foodborne pathogens can be present in a variety of foods, and it is critical to detect them in order to ensure a safe food supply and prevent foodborne illnesses. Traditional methods for detecting foodborne pathogens are time-consuming and labor-intensive. As a result, a range of technologies for quick detection of foodborne pathogens have been developed, as it is necessary for many food analysis. Nucleic acid-based, biosensor-based, and immunological-based approaches are the three types of rapid detection methods. The ideas and use of modern quick technologies for the detection of foodborne bacterial infections are the focus of this chapter.
Costus igneus plants are highly active against the majority of Gram-negative and Gram-positive microorganisms. The purpose of the current study was intended to evaluate the anti-microbial potential of methanolic extract of Costus igneus over multidrug-resistant bacteria, specifically to methicillin, vancomycin, carbapenems, colistin. The study also focused on the antifungal activity of the plant extract against Candida species. Phytochemical analysis was conducted to identify the presence of the active chemicals such as steroids, alkaloid, flavonoids, polyphenols, terpenoids, saponin, tannin, glycosides, quinones, coumarins and phenolic compounds using standard protocols. Anti-microbial activity of C. igneus was assessed through agar well diffusion technique and Minimum inhibitory concentration method (MIC) by using multidrug-resistant Gram-positive microorganisms (Staphylococcus aureus, Enterococcus faecalis) and multidrug-resistant Gram-negative microorganisms (E.coli, Klebsiella pneumonia, Pseudomonas aeruginosa, Salmonella typhi, Proteus mirabilis, Citrobacter species) and also Candida albicans. At 120mg/ml C.igneus plant extract concentration, maximum zone of inhibition was obtained with all the nine tested microorganisms and however the zone of inhibition was slighter with regular standard potential antibiotics like colistin, imipenem etc. Anti-microbials of plant origin possesses tremendous therapeutic potential as they can accomplish the requirements with fewer sideeffects that are routinely associated with synthetic anti-microbials. In this investigation, it was established that C. igneus leaf extract possesses excellent anti-microbial activity which can be attributed to the occurrence of phytochemicals. Further discovery of plant-derived anti-microbials should be continued to trim down the usage of powerful drugs which pose bad side effects.
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