INTRODUCTION: The main objective of the present work was to develop a simple, precise, specific & stability indicating RP-HPLC method for simultaneous estimation of Teneligliptin and Metformin in bulk and tablet dosage form. METHODS: The analysis has been performed using Kromasil C18 column (250 x 4.6 mm, 5µ) at 30°C using buffer: acetonitrile: methanol (65: 25: 10, v/v/v) as mobile phase. The detection was carried out at 254 nm with a flow rate of 1.0 ml/min. RESULTS: The retention time of Teneligliptin and Metformin was found to be 2.842 min & 2.017 min respectively. The linearity range was 5-30 µg/ml for Teneligliptin and 125-750 µg/ml for Metformin respectively. The forced degradation studies were performed as per the guidelines of ICH under acidic, alkaline, oxidative, thermal, photo stability & neutral conditions. DISCUSSION AND CONCLUSION: The developed method was successfully validated for all the parameters and was found to be within the limits. The developed method could be successfully employed for the simultaneous estimation of Teneligliptin and Metformin in bulk and tablet dosage form.
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