Chronic exposure to stress and diet rich in saturated fat is one of the major reasons for the development of dementia and neurodegenerative disorders. The present study aims to examine the neuroprotective potential of and Ascorbic acid against high fat diet and stress induced neurotoxicity in brain. Animals were randomly divided into five groups. Group I received normal diet, Group II received high fat diet along with stress, Group III were treated with 100mg/kg body weight, and Group IV were treated with Ascorbic acid 100mg/kg body weight, Group V were treated with 100mg/kg body weight and Ascorbic acid 100mg/kg body weight. After the treatment all rats were sacrificed and brains were removed. Golgi staining was done and dendritic branching points and dendritic intersections were quantified with the help of cameralucida.There was a significant increase in dendritic length and branching points was observed in brain in rats treated with and Ascorbic acid.Present study concludes that and Ascorbic acid have neuroprotective role against high fat diet and stress induced Wistar rats.

Back ground: Prolonged stress and diet rich in cholesterol is known to cause memory impairment and cause disruption of neuronal net work in hippocampus. In the present study, we evaluated the neuroprotective effect of rosuvastatin HMG CoA reductase inhibitor on stress induced and high fat diet fed rats given in combination.Materials & methods: Forty eight adult male wistar rats were randomly assigned into eight groups. (N=6) control group received normal diet (chow diet), second group received only high fat diet (HFD), third group received only stress (STS), fourth group high fat diet and stress (HFD+STS), fifth group control + rosuvastatin 10mg/body wt, sixth group control + rosuvastatin 20mg/body.wt, seventh group received high fat diet + stress and treated with rosuvastatin10mg/body.wt (HFD+STS+ROS 10mg), eighth group was treated with rosuvastatin 20mg/body.wt (HFD+STS+ROS 20mg).Spatial memory was assessed by morris water maze. Rats were sacrificed at 96 th day; brains were removed and processed for histological studies using cresyl violet staining. Neuronal population of CA1 &CA3 region was quantified. Results: A significant (p < 0.01) increase in the neuronal population in the sub regions of hippocampus and improvement of spatial memory (CA1:32± 1.95, CA3: 22± 1.5, latency to enter the target quadrant LT: 6.05±0.9sec, time spent in target quadrant TST: 14.4± 1.62 sec was seen in rats treated with rosuvastatin 20mg/kg.body.wt (HFD+STS+ROS 20mg) compared to high fat diet and stress (HFD+STS): (CA1: 28±1.33,CA3 :18± 1.47 & LT: 7.67 ± 0.77 sec, TST: 10 ± 1.37 sec. The rats treated with 10mg/kg.body. wt (CA1:30±1.83, CA3:22± 1.37) did not show any significance compared to HFD+STS group. There was also significant (p < 0.01) improvement in only high fat group treated with rosuvastatin 20mg/kg.body.wt (HFD+ROS-20mg) CA1:33.

Diet rich in fat is one of the main risk factor for the development of Alzheimer’s disease. Studies have shown that diet rich in fat disrupts memory and learning. The present study evaluates the ameliorative role of Ginkgobiloba and Rosuvastatin against high fat diet induced neurotoxicity in CA1 (Corona Ammonis) region of hippocampus. Animals were randomly divided into six groups. Group I received normal diet, Group II received high fat diet, Group III & IV were treated with Ginkgobiloba 50mg/kg and 100mg/kg body weight, and Group V & VI were treated with Rosuvastatin 10mg/kg and 20 mg/kg body weight. All the rats were subjected to spatial learning (Morris water maze). Subsequently, rats were sacrificed and brains were removed. Golgi staining was done and CA1 neurons of hippocampus were traced using camera lucida. Dendritic branching points and dendritic intersections were quantified. Lipid profile and Super oxide (SOD) was also estimated.There was enhancement of spatial learning in treatment group rats. Furthermore, a significant increase in dendritic length and branching points was observed in CA1 region along with significant decrease in the Superoxide dismutase in rats treated with higher dose of Ginkgobiloba and Rosuvastatin. Present study concludes that Ginkgobiloba and Rosuvastatin in higher dose have protective role against high fat diet induced neurotoxicity in CA1 region.