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Tannins are polyphenolic compounds found to exhibit antibacterial, antiviral and antioxidant property. This paper represents an analytical approach to characterize tannins, phenolic compounds and to assess the

Objective: The idea of green chemistry has gained immense fame due to replace chemical products and improves technologies to eradicate substances that are harmful to the environment. In this paper, a rapid cost-efficient method was employed using herbal extract Gymnema sylvestrae because of their biological constituents present in the sample. Methods: Phytosynthesis of AgNPs were optimized under different reaction conditions using pH, temperature, incubated at various concentrations. Analyses of particles were revealed using UV-Vis, FTIR spectrum, morphology was observed in scanning electron microscope, particle analysis was done using Diffraction Light Scattering and bioactive constituents present in plant sample was analysed by High-performance liquid chromatography. Bioefficacy of synthesised AgNPs was assessed by means of microbicidal assay against various bacteria and fungi. Results: UV and FTIR analysis reveals the presence of plant extract responsible for stabilization and efficient reduction. Peptides to proteins, polyphenols, and many other secondary metabolites involved in the bioreduction were identified. SEM micrograph reveals the nature, size and distribution of the sample. HPLC chromatogram indicated the presence of gymnemagenin responsible for their biological assays. Broad spectrum of microbicidal activity have been reported in 400 µl of biosynthesized AgNPs against Bacillus sp. (24.5 mm), and S. epidermis (22.3 mm). Conclusion: Therefore G. sylvestrae synthesized silver nanoparticles were stable and acts as a reducing and capping agent detecting the presence of biomolecules. Biosynthesised AgNPs showing excellent antimicrobial activity and future prospects of this study indicates that these nanoparticles can be applied in drug delivery.

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Nanotechnology includes physical and chemical approaches intended for their phytofabrication of metal nanoparticles. Most of the time, these methods are not as safe as these are associated with the utilization of extremely noxious and hazardous substances that produce biological problems. Due to their manifold applications, various biological methods are gaining admiration for the fabrication of silver nanoparticles (AgNPs). The utilization of vegetative parts in the biosynthesis of nanoparticles (NPs) emerges as a commercial and ecological approach. The spectroscopic profile confirms the occurrence of a functional peak at 360 nm. Fourier transform infrared spectroscopy (FTIR) studies indicate that phenol groups are responsible for phytofabrication of NPs. The results of microbicidal screening confirm that broad spectrum of inhibition was found to be observed in 400 μL of biosynthesized AgNP against E. coli (23 mm) and B. cereus (22.3 mm) Therefore, the progress of simple photosynthesis methods avoid deleterious and harmful properties has fascinated major attention in this field.