Objective: Endophytic fungi live inside the higher plants, apparently without causing any harm to the hosts and its produce the secondary metabolites are potential antimicrobial activity. Terminalia chebula has been used in Ayurveda, Unani, and Homeopathy medicine. In this study, an isolate and identify the endophytic fungi from T. chebula collected from Pachamalai hills of the Eastern Ghats, Tamil Nadu. Methods:The plant materials were taken and first rinsed in running tap water to remove the dust and the other debris present in it. Segments of approximately 0.5 cm were cut in sterile lancet blades and surface sterilized by agitating in 70% ethanol (5 s), followed by treatment with 4% NaOCl (90 s) and then rinsed in sterile distilled water (10 s). 36 (leaf, stem and fruit samples) segments from T. chebula plant are processed for the isolation of endophytic fungi.Results: About 36 segments (12 segments of each part respectively) of the medicinal plant were screened for the isolation of the endophytic fungi. A total of 27 endophytic fungi was isolated and identified from medicinal plant T. chebula. The leaf segments showed a maximum repository for endophytic fungi than the other segments. Among the 27 endophytic fungi, the predominant endophytic fungi isolated belonged to the genera of Alternaria longipes, Curvularia spp., Mucor phoma spp., Aspergillus niger, Aspergillus flavus, and Penicillium spp. In this study, the majority of the fungi belonged to hyaline hyphomycetes. Conclusion:In this study, conclude that the isolation of endophytic fungi from medicinal plant of T. chebula. To isolate the 27 endophytic fungi produce the novel bioactive compound. However, further studies are required to screen these endophytic fungi for production of novel bioactive compounds.