A pot culture experiment in the factorial completely randomized design (FRCD) was formulated with the three levels of iron (Fe) @ 0, 20 and 40 mg kg-1 in the iron-deficient Typic Ustochrept sandy loam soil at Anand Agricultural University, Gujarat, India to evaluate and predict the iron uptake, nutrient efficiency and its depletion of in the rhizosphere of efficient and in-efficient chick pea cultivars using mechanistic model NST 3.0. The current investigation was carried out with the four chickpea cultivars namely, Fe-efficient (GG-1 and GAG-735) and Fe-inefficient (ICCC-4 and GJG-305). Plant observations were recorded during three different growth stages viz., 20 DAG, 40 DAG and maturity, respectively. The shoot weight and shoot length of Fe-inefficient varieties (ICCC-4 and GJG-305) well responded to the application of Fe as compared to Fe-efficient varieties (GG-1 and GAG-735) with 20 mg Fe Kg-1 application through FeSO4. Lower dose of 20 mg Fe kg-1 was found equally effective in increasing root length and root radius. Root radius (ro) and initial soil solution concentration of Fe (CLi) were found most sensitive parameters influencing Fe uptake, which was followed by maximum net influx (Imax). In no Fe treatment, increasing r0, CLi by a factor of 2.0 times individually caused increase in Fe uptake by 1.60, 1.45 times, 1.36, 1.53 times, 1.16, 1.15 times and 1.05, 1.25 times, respectively in GG-1, GAG-735, ICCC-4 and GJG-305 varieties of chickpea. While, increasing Imax and Km separately by a factor of 2.0 Fe uptake altered in proportions by 1.03, 0.57 times, 0.93, 0.57 times, 0.73, 0.54 times and 0.69 and 0.48 times, respectively in GG-1, GAG-735, ICCC-4 and GJG-305 varieties of chickpea. The ICCC-4 instead of GG-1 and GAG-735 could be rational choice to grow on Fe deficient soil to get with dense Fe content. On the other hand, Fe-Inefficient varieties had 2 times of higher mean Fe-influx at 40 mg Fe kg-1 application than Fe-Efficient varieties.

In this present investigation, two Multi Antibiotics Resistant (MAR) bacterial strains namely SR2 and SR4 were isolated from clinical waste. The bacterial isolate SR2 was resistant to most of the antibiotics tested, but sensitive to levofloxacin. While, the other strain SR4 was sensitive to Cefixime and levofloxacin. Morphological, biochemical and 16S rRNA sequence analysis identified these 2 bacterial strains SR2 and SR4 as Acinetobacter sp. (GenBank Acc. No. KJ879241) and Aeromonas hydrophila (GenBank Acc. No. KJ879242), respectively. The conjugal transfer efficiency of antibiotics resistant gene to another bacterial strain was also tested, using Corynebacterium alkanolyticum ATH3 as a recipient. In order to control these pathogenic bacterial strains, leaf extracts obtained from three Cassia plants named C. siamea, C. allata and C. occidentalis were used at different concentration. Cassia siamea was recorded to be most effective against both of these bacterial strains. Growth kinetics of these two bacterial strains revealed that growth was decreased with gradual increase of concentration of leaf extract, obtained from C. siamea and ultimately negligible growth at 30 mg mLG 1 (3%).