Abstract. thiopurine S-methyltransferase (tpmt) catalyzes the S-methylation of aromatic and heterocyclic sulfhydryl compounds including thiopurine drugs such as 6-mercaptopurine, 6-thioguanine and azathioprine. tpmt activity exhibits genetic variation and shows tri-modal distribution with 89-94% of individuals possessing high activity, 6-11% intermediate activity and approximately 0.3% low activity. Patients with intermediate or deficient TPMT activity exposed to thiopurine drugs show severe hematopoietic toxicity. three single nucleotide polymorphisms (Snps) in TPMT (nm_000367.2:c.238G>c, nm_000367.2:c.460G>a and NM_000367.2:c.719A>G) define the most prevalent mutant alleles associated with loss of catalytic activity reported in several populations. The present study investigated, for the first time, the frequency distribution of these three Snps of TPMT, their alleles and genotypes in a Southern indian population. peripheral blood was obtained from 326 individuals of a Southern indian population, and genomic dna was isolated from total peripheral white blood cells. the genotypes at the polymorphic loci were determined by allele-specific polymerase chain reaction, restriction fragment length polymorphism and confirmatory DNA sequencing. The estimated genotype frequency for homozygous TPMT

No abstract

No abstract

INTRODCUTION:Cardiovascular diseases account for serious health issues, which results in loss of quality of life andhigh mortality and morbidity. Cardiovascular disease risk in India is greater than US and Europe. Among various causative factors, Type 2 diabetes mellitus (T2DM) is one of the most prevalent metabolic diseases associated with increasedrisk for CAD. METHODOLOGY:In this study, we assess the clinical and demographic parameters along with hsCRP levels between the group of participants with 1) T2DM 2) T2DM with CAD compared with healthy controls. All participants were subjected to basic clinical laboratory test like Blood pressures (systolic and diastolic), lipid profile, HbA1c level (glycosylated hemoglobin), FBS and PPBS. Additionally, the enzyme-linked immune absorbent assay was used to determine the levels of serum hs-CRP in all the participants recruited for this study.RESULTS: Analysis of clinical characteristics showed significant association of lipid, HbA1c, and hsCRP levels in the participants with T2DM with CAD contributing towards cardiovascular disease. Particularly, the level of hsCRP along with HbA1c maycontribute detecting early CVD event in participants with type 2 diabetic mellitus. CONCLUSION:Overall, our study provides significant contribution in predication of early CVD event in T2DM Murugesan et al (2020): Cardiac biomarkers in type II diabetic patients