First order derivative spectrophotometric method and high performance liquid chromatographic method were developed for the determination of Triprolidine and Pseudoephedrine Hydrochloride in tablet dosage form. In UV-Spectrophotometric method, estimation of Triprolidine and Pseudoephedrine Hydrochloride was carried out at the wavelength selected 246.20 nm and 263.50 nm for First order Derivative method. Calibration curves were linear in the range of 2-10 μg ml-1 for Triprolidine and 48-240 μg ml-1 for Pseudoephedrine Hydrochloride in derivative method. Correlation coefficient found to be close to 0.9950 for both the drugs. Accuracy for both the drugs was in the range of 99-101.5%. A simple liquid chromatographic assay has been developed for the determination of Triprolidine and Pseudoephedrine Hydrochloride. A C 18 (250×4.6 mm, 5 μm) column was used with a mobile phase consisting of Methanol: Water (80: 20 v/v) (pH adjusted to 3.0 with ortho phosphoric acid) at a flow rate of 1.0 ml min-1. Quantitation was achieved with UV detection at 246.20 nm based on the peak height ratios. Beer's law was obeyed in a concentration range of 5-25 µg ml-1 for Triprolidine and 120-600 µg ml-1 for Pseudoephedrine Hydrochloride and the regression line equation was derived with a correlation coefficient of 0.9999 and 0.9998 for Triprolidine and Pseudoephedrine Hydrochloride. The proposed procedures were successfully applied to the determination of Triprolidine and Pseudoephedrine Hydrochloride in bulk and tablet form, with high percentage of recovery, good accuracy and precision.

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Absorbance ratio spectrophotometric method and high performance liquid chromatographic method were developed for the determination of the Ofloxacin and Azithromycin in tablet dosage form. In UV-Spectrophotometric method, estimation of Ofloxacin and Azithromycin was carried out at 230.60 nm (isoabsorptive point) and 288.60 nm (λ max of Ofloxacin) for absorbance ratio method. Calibration curves were linear in the range of 4-20 μg/ml for Ofloxacin and 5-25 μg/ml Azithromycin for absorbance ratio method. Correlation coefficient found to be close to 0.9995 for both the drugs`. Accuracy for both the drugs was in the range of 98-101%. A simple liquid chromatographic assay has been developed for the determination of Ofloxacin and Azithromycin. A C 18 (250 mm × 4.6 mm, 5 μ) column was used with a mobile phase consisting of Phosphate buffer: Methanol (pH adjusted to 5.0 with ortho phosphoric acid) at a flow rate of 1.0 ml min-1. Quantitation was achieved with UV detection at 215 nm based on the peak height ratios. Beer's law was obeyed in a concentration range of 12-28 µg ml-1 for Ofloxacin and 15-35 µg ml-1 for Azithromycin and the regression line equation was derived with a correlation coefficient of 0.9970 and 0.9980 for Ofloxacin and Azithromycin respectively. The validity of the methods was further confirmed using the standard addition method. The proposed procedures were successfully applied to the determination of Ofloxacin and Azithromycin in bulk and tablet form, with high percentage of recovery, good accuracy and precision.

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