Staphylococcus aureus is one of the most dangerous organisms that threatens the life of humans and animals alike. It has been found that people who suffer from chronic diseases such as diabetes, blood vessels, pulmonary and cancerous diseases are more susceptible to infection, in addition to ability resist antibiotics, which made fierce and dangerous. This staphylococcus aureus possessed the SAK gene, which acts as a bio stimulant that plays an important role in the process of analyzing blood coagulation, as it acts as an anti-plasmogen activator and is in the form of an equal compound of human plasmin. SAK consists of a chain of amino acids numbering 136 amino acids. The study aimed to isolate the SAK gene using amplification technique PCR and insert it into an ideal vectorPET21a. And he transferred it to one of the strains of the E.coli(BL21DE3) , stimulating the bacteria by adding IPTG, the purpose of obtaining the highest level of protein expression in an ideal time, then purifying the protein by affinity chromatography.

Thermal conductivity of soil is one of the phenomenon which measure the amount of heat passing per cross-sectional area. Thermal conductivity of soils depends on various factors. In this study the influence of presence of Microorganisms on enhancement of thermal conductivity of soils is determined experimentally. In this research, soil samples from four different regions namely Dholera, Navsari, Sabarmati River and Madhavpur of Gujarat, India, were taken. The aim of study was to develop experimental model to measure the enhancement of thermal conductivity of soil by microorganisms. The laboratory model which measures thermal conductivity of soil is based on guarded hot plate method. The colonies of microorganisms available in soil were studied by serial dilution and spread plate technique. The influence of microorganisms on soil structure after the treatment was analysed by microscope. Four types of microorganisms were introduced in the soil and the thermal conductivity was recorded after one week of curing period. The microorganisms which gave better result of thermal conductivity were selected for further study. Thereafter, thermal conductivity of sample was measured for curing period of 7, 14, 21 and 28 d. The results of thermal conductivity and soil aggregations were compared after pre and post treatment.

A previous research and studies have established the significance of describing Staphylokinase as a thrombolytic treatment, particularly in patients with cardiovascular disease, stroke, and other life-threatening disorders. Staphylokinase plays a significant part in the coagulation process through formation plasmin-Staphylokinase complex on the surface of the clot which activates plasminogen is generated by some strains of Staphylococcus aureus. The publication described the use of the E. coli strain BL12DE3 to produce the protein via the pET21a transporter is predicated on the use of a chemical stimulator (IPTG) which plays an important role in regulating protein recombinant by stimulating protein synthesis. Strain BL12DE3 activates T7 polymerase, encoding LacI via the pET21a transporter. It has been demonstrated that the IPTG inducer achieves the maximum level of protein expression in the shortest amount of time. A Staphylokinase was generated as a soluble protein in excess of 45 % by SDS-PAGE and subsequently purified by chromatography.

A previous research and studies have established the significance of describing Staphylokinase as a thrombolytic treatment, particularly in patients with cardiovascular disease, stroke, and other life-threatening disorders. Staphylokinase plays a significant part in the coagulation process through formation plasmin-Staphylokinase complex on the surface of the clot which activates plasminogen is generated by some strains of Staphylococcus aureus. The publication described the use of the E. coli strain BL12DE3 to produce the protein via the pET21a transporter is predicated on the use of a chemical stimulator (IPTG) which plays an important role in regulating protein recombinant by stimulating protein synthesis. Strain BL12DE3 activates T7 polymerase, encoding LacI via the pET21a transporter. It has been demonstrated that the IPTG inducer achieves the maximum level of protein expression in the shortest amount of time. A Staphylokinase was generated as a soluble protein in excess of 45% by SDS-PAGE and subsequently purified by chromatography.