A method is dcscribcd for the rapid dctcrmination of dissolved organic carbon in seawater in concentrations bctwcen 0.1 and 20 mg/liter. The oxidation is carried out in sealed glass ampoules using K&Lox as an oxidizing agent after the sample has been freed of inorganic carbon. The resulting CO2 is passed through a nondispcrsive infrared analyzer using nitrogen as a carrier and the signal output of the analyzer recorded, Using appropriate calibration curves, the carbon content is determined From the height of the peak. Approximately 100 samples can bc analyzed in a single clay with a precision of -I-0.1 mg/liter using a sample volume of 5 ml. A companion method is described for the determination of particulate carbon by hightemperature combustion after concentration of the sample on a glass-fiber filter.The precision of this method is -c-10 mg C in a range of O-500 pg C. Approximately six samples can be analyzed in an hour. INTRODUCTCON
To test the applicability of substrate uptake kinetics for assessing rates of microbial heterotrophic activity in the sea, the conformity between accepted notations for pure enzyme systems as opposed to natural populations is examined.
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