In continuation of our work on the plant Echinops echinotus Roxb. (Compositae) (1), we report here the isolation of apigenin, apigenin 7-O-glucoside, and a new acylfiavone glucoside from the flowers of E. echinatus. The ethyl acetate fraction of the alcoholic extract of the flowers (3kg) of Echinops echinatus was chromatographed over an SiO2 gel column. The C6H6-EtOAc (1:1) and EtOAc eluates furnished apigenin (22 mg) and its 7-O-glucoside (31 mg) and the EtOAc-MeOH (20: 1) eluates gave light yellow granules of an acylfiavone glucoside (74 mg) designated as echitin (1).