Mucosal-associated invariant T (MAIT) cells, defined as CD161++TCR iVα7.2+ T cells, play an important role in the innate defense against bacterial infections, and their functionality is impaired in chronic viral infections. Here, we investigated the frequency and functional role of MAIT cells in chronic hepatitis B virus (HBV) infection. The peripheral CD3+CD161++TCR iVα7.2+ MAIT cells in chronic HBV-infected patients and healthy controls were phenotypically characterized based on CD57, PD-1, TIM-3, and CTLA-4, as well as HLA-DR and CD38 expression. The frequency of MAIT cells was significantly decreased among chronic HBV-infected individuals as compared to controls. Expression of CD57, PD-1, CTLA-4, as well as HLA-DR and CD38 on MAIT cells was significantly elevated in chronic HBV-infected individuals relative to controls. The percentage of T cell receptor (TCR) iVα7.2+ CD161+ MAIT cells did not correlate with HBV viral load but inversely with HLA-DR on CD4+ T cells and MAIT cells and with CD57 on CD8+ T cells suggesting that decrease of MAIT cells may not be attributed to direct infection by HBV but driven by HBV-induced chronic immune activation. The percentage and expression levels of PD-1 as well as CTLA-4 on MAIT cells inversely correlated with plasma HBV-DNA levels, which may suggest either a role for MAIT cells in the control of HBV infection or the effect of HBV replication in the liver on MAIT cell phenotype. We report that decrease of TCR iVα7.2+ MAIT cells in the peripheral blood and their functions were seemingly impaired in chronic HBV-infected patients likely because of the increased expression of PD-1.

Tuberculosis (TB) and human immunodeficiency virus (HIV) infection interfere and impact the pathogenesis phenomena of each other. Owing to atypical clinical presentations and diagnostic complications, HIV/TB co-infection continues to be a menace for healthcare providers. Although the increased access to highly active antiretroviral therapy (HAART) has led to a reduction in HIV-associated opportunistic infections and mortality, the concurrent management of HIV/TB co-infection remains a challenge owing to adverse effects, complex drug interactions, overlapping toxicities and tuberculosis -associated immune reconstitution inflammatory syndrome. Several hypotheses have been put forward for the exacerbation of tuberculosis by HIV and vice versa supported by immunological studies. Discussion on the mechanisms produced by infectious cofactors with impact on disease pathology could shed light on how to design potential interventions that could decelerate disease progression. With no vaccine for HIV and lack of an effective vaccine for tuberculosis, it is essential to design strategies against HIV-TB co-infection.

The extraction of three-dimensional shape from shading is one of the most perceptually compelling, yet poorly understood, aspects of visual perception. In this paper, we report several new experiments on the manner in which the perception of shape from shading interacts with other visual processes such as perceptual grouping, preattentive search C'pop-out"), and motion perception. Our specific findings are as follows: (1) The extraction of shape from shading information incorporates at least two "assumptions" or constraints-first, that there is a single light source illuminating the whole scene, and second, that the light is shining from "above" in relation to retinal coordinates. (2) Tokens defined by shading can serve as a basis for perceptual grouping and segregation. (3) Reaction time for detecting a single convex shape does not increase with the number of items in the display. This "pop-out" effect must be based on shading rather than on differences in luminance polarity, since neither left-right differences nor step changes in luminance resulted in pop-out. (4) When the subjects were experienced, there were no search asymmetries for convex as opposed to concave tokens, but when the subjects were naive, cavities were much easier to detect than convex shapes. (5) The extraction of shape from shading can also provide an input to motion perception. And finally, (6) the assumption of "overhead illumination" that leads to perceptual grouping depends primarily on retinal rather than on "phenomenal" or gravitational coordinates. Taken collectively, these findings imply that the extraction of shape from shading is an "early" visual process that occurs prior to perceptual grouping, motion perception, and vestibular (as well as "cognitive") correction for head tilt. Hence, there may be neural elements very early in visual processing that are specialized for the extraction of shape from shading. We use three-dimensional (3-D) depth perception to find our way around the world and to manipulate objects that we encounter. Although the retinal image is two-dimensional, somehow the brain is able to use the information from this image to yield an experience of solidity and depth. Of the numerous mechanisms used by the visual system to recover the third dimension, the ability to use shading is probably phylogenetically one of the most primitive. One reason for believing this is that in the natural world, animals have often evolved the principle of countershading to conceal their shapes from predators; they have pale bellies that serve to neutralize the effects of the sun shining from above (Thayer, 1909). The prevalence of countershading in a variety of animals (including fishes) suggests that shading must be a very important source of information about 3-D shapes. Although artists have long recognized the importance of shading, there have been few studies of how the human visual system actually extracts and uses this information. Since the time when Leonardo da Vinci first

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