No abstract

BackgroundGlycemic response, a trait that is tedious to be assayed in cereal staples, has been identified as a factor correlated with alarmingly increasing prevalence of Type II diabetes. Reverse genetics based discovery of allelic variants associated with this nutritional trait gains significance as they can provide scope for genetic improvement of this factor which is otherwise difficult to target through routine screening methods.ResultsThrough EcoTILLING by sequencing in 512 rice accessions, we report the discovery of six deleterious variants in the genes with potential to increase Resistant Starch (RS) and reduce Hydrolysis Index (HI) of starch. By deconvolution of the variant harbouring EcoTILLING DNA pools, we discovered accessions with a minimum of one to a maximum of three deleterious allelic variants in the candidate genes.ConclusionsThrough biochemical assays, we confirmed the potential role of the discovered alleles alone or in combinations in increasing RS the key factor for reduction in glycemic response.Electronic supplementary materialThe online version of this article (doi:10.1186/s12870-016-0968-0) contains supplementary material, which is available to authorized users.

No abstract

High performance liquid chromatography (HPLC) was performed to determine the raffinose family oligosaccharides (RFOs) and sucrose content in 32 pigeonpea cultivars. The results showed that the RFOs in the pigeonpea seed extracts comprised of raffinose, stachyose and verbascose. The contents of raffinose, stachyose and verbascose were within the range of 1.15 to 2.55%, 2.25 to 4.05% and 2.05 to 4.05% with mean values of 1.63%, 2.97% and 2.80%, respectively. There was large level of variation of individual RFOs. Furthermore, to study the effect of post-harvest processing on the RFO contents, sprouting and cooking experiments were conducted with the seeds of the variety VBN 3 which possessed high RFO levels. Sprouting significantly reduced the RFO levels within 24 hours while cooking even up to 30 min in boiling water did not result in a considerable reduction. Among the local processing techniques, sprouting had significant effect on reducing RFOs content in pigeonpea.