Endometrial pathologies have been a predominate problem faced by women globally.Among all the endometrial pathologies, abnormal uterine bleeding is first in the list among the developing countries. To analyse the percentage of endometrial pathology detected in women who were diagnosed from abnormal uterine bleeding in our tertiary health care centre.A retrospective study of 158 women patients who were diagnosed with abnormal uterine bleeding and their endometrial tissue sampling were evaluated for endometrial pathology. The study data were collected from February 2017 till January 2019 based on Inclusion criteria and Exclusion criteria.According to the inclusion criteria, the 152 cases medical data which includes clinical evaluations along with endometrial histopathology evaluation were analysed. According to our analysis, the histopathological examination reports revealed most of the cases were diagnosed with benign endometrial pathology (34.72%) and abnormal endometrial pathology (9.09%). The 9.09% includes 7.44% presented with endometrial hyperplasia and 1.65% presented with endometrial cancer.Histopathological study of endometrial curettage tissue is the approved method for detecting any endometrial pathology. This is an onerous task for pathologists as not to miss any important findings. According to our analysis the commonest incidences of endometrial pathology were normal endometrium 56.19% of cases and benign pathology 34.72% and the less incidences of preneoplastic lesions 7.44% and endometrial carcinoma 1.65%.
Introduction: Stains are a crucial component of laboratory procedure. They help in highlighting the different tissues, both normal and abnormal, which plays an important role in diagnostic histopathology. Synthetic dyes are utilised for majority of the stains in histology. Among the natural surrogates studied, Kumkum is less researched. Aim: To compare the staining characteristics of Kumkum, which imparts red colour as an alternative to eosin. Materials and Methods: This observational study was conducted at Histopathology section, Pathology department at Chettinad Hospital and Research Institute for a period of six months from January 2022 to June 2022. In 50 tissue blocks, each was made into two sections and one was stained with routine Haematoxylin and Eosin (H&E), while the other was stained with Haematoxylin and Kumkum solution (H&K). The Cellular Architecture (CA) (based on distinct or indistinct nucleus, cytoplasm) and quality of staining (poor, satisfactory or good) of H&E and H&K solution was compared and a scoring system was given. The scores were analysed with Chi-square test using SPSS software (version 25). Results: Out of 100 slides, 45 (90%) stained with H&E and 48 (96%) with H&K showed distinct CA. A total of 47 slides (94%) stained with H&K and 41 (82%) with H&E showed good quality of staining. Conclusion: Kumkum solution appears to be an efficient counterstain in place of eosin in highlighting the normal structures in histopathological sections. It highlighted the RBCs in the arteries and cytoplasm of the cells in glandular and squamous epithelium better than or equally good as eosin.
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