This study aimed to reveal the level of burnout among the faculty members in private universities in Jordan and the effect of gender and experience variables on it. The researchers used the descriptive analytical method. The study was applied to a random sample of (203) faculty members, who were chosen through a comprehensive survey method, with (169) males and (34) females. A job burnout questionnaire was developed, and its validity and reliability were verified. The results of the study showed that the mean of the job burnout of the total degree came with a high degree. The results also showed that there were no statistically significant differences in the level of job burnout of the total degree attributed to the gender variable and in favor of females. Moreover, the results showed that there were apparent differences in the arithmetic averages on the total score for the level of job burnout with different levels of experience variable, and in favor of those with less than five years of experience.