A new acylated flavone glycoside, echioidinin 2'-O-beta-D-(2''-O-acetyl)glucopyranoside (1) along with the three known flavonoids, 5,6,7,8,5'-pentamethoxy-3',4'-methylenedioxyflavone (2), 5,2',6'-trihydroxy-7-methoxyflavone (3), and kaempferol 7,4'-dimethyl ether 3-O-beta-D-glucopyranoside (4), were isolated from the roots of Colebrookea oppositifolia. The structure of compound 1 was elucidated by extensive spectral and chemical studies.
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