Objective:The objective of this study was to compare the elastic modulus between milled, 3D printed and conventional compression moulded denture base resins (DBRs). Materials and methods: Three different types of DBRs were used: milled resin (pre-polymerized PMMA plates) ; a 3D printed resin (photopolymerized resin); and a polymethyl methacrylate (PMMA) heat cured resin (powder-liquid system). Thirty specimens have been constructed with specific dimensions (65mm x10mm x3mm) and divided into 3 groups (10 for each group) according to the type of DBR, Group I contained the milled DBR specimens, Group II contained 3-dimentional printed DBR specimens, and Group III contained conventional compression moulded DBR specimens. The elastic modulus of the 30 specimens were measured and calculated by universal testing machine using three-point loading test. Results: The elastic modulus of the milled group was significantly higher than that of the other 2 groups (P<0.05), while the elastic modulus of the compression moulded group was significantly greater than that of the 3D printed group (P<0.05). Conclusion: milled DBR show the highest elastic modulus when compared with conventional compression moulded or 3D printed DBRs, while 3D printed DBR shows the lowest elastic modulus.