Nanoparticles can be synthesized by chemical, physical and biological system methods. In this work, Superparamagnetic Iron Oxide@Silver nanoparticles (SPION@Ag NPs) were modified by zinc oxide nanoparticles (ZnO NPs). SPION@Ag core shell NPs were synthesized by the biological method using Fusarium oxysporum fungus and coated with ZnO NPs. This method is eco-friendly and low cost. Characterization techniques such as UV, FTIR, HR-TEM, HR-SEM, XRD and zeta potential were used. The most common type of liver cancer is hepatocellular carcinoma (HCC). Hepatocellular carcinoma was induced by a single dose of diethyl nitrosamine (DEN) 60 mg/Kg b.wt. and was followed after two days by carbon tetra chloride (CCl 4 ) diluted with paraffin oil (50% v/v, 2 ml/Kg b.wt.) twice a week for one month. A histopathological examination was done after sacrifying. Clear changes were shown in the comparison between the HCC group treated with ZnO@SPION@Ag nanocomposite and the positive control group. Liver function tests showed a highly significant decrease after using the nanocomposite for HCC treatment. Our study aim is to evaluate the therapeutic anticancer effect of ZnO@SPION@Ag nanocomposite on hepatocellular carcinoma in male albino rats, which was synthesized by a new method called green chemistry or green synthesis.

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