The present article reviews the diagnosis of Novel Corona Virus Disease (COVID-19) by using Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR). COVID-19 is a contagious disease caused by severe acute respiratory syndrome coronavirus2 (SARS-CoV-2), which affects upper and lower respiratory tract. In present scenario COVID-19 is considered as global public health emergency by World Health Organization (WHO) so, the detection and prevention of this disease is vital to control this emergency. RT-PCR is a highly sensitive laboratory method for the detection of SARS-CoV-2. It is based on rapid diagnosis of SARS-CoV-2 by qualitative detection of its nucleic acid sequence. This test locates the primer and probe sets in different regions in the genome of SARS-CoV-2.