Silicon (Si) is known to alleviate many nutritional stresses. However, in Brassica napus, which is a highly S-demanding species, the Si effect on S deficiency remains undocumented. The aim of this study was to assess whether Si alleviates the negative effects of S deficiency on Brassica napus and modulates root sulfate uptake capacity and S accumulation. For this, Brassica napus plants were cultivated with or without S and supplied or not supplied with Si. The effects of Si on S content, growth, expression of sulfate transporter genes (BnaSultr1.1; BnaSultr1.2) and sulfate transporters activity in roots were monitored. Si supply did not mitigate growth or S status alterations due to S deprivation but moderated the expression of BnaSultr1.1 in S-deprived plants without affecting the activity of root sulfate transporters. The effects of Si on the amount of S taken-up and on S transporter gene expression were also evaluated after 72 h of S resupply. In S-deprived plants, S re-feeding led to a strong decrease in the expression of both S transporter genes as expected, except in Si-treated plants where BnaSultr1.1 expression was maintained over time. This result is discussed in relation to the similar amount of S accumulated regardless of the Si treatment.

Optimizing nitrogen use efficiency (NUE) could mitigate the adverse effects of nitrogen (N) fertilizers by limiting their environmental risks and raising agronomic performance. We studied the effects of VNT4, a derived formulation of Glutacetine® biostimulant, mixed with urea-ammonium-nitrate solution (UAN) on the growth, N-related traits and agronomic performance of winter wheat (Triticum aestivum L.). The experiment was performed under six contrasting field conditions over two years in Normandy (France), including a site where 15N labelling was undertaken. Taking into account all the sites, we report that VNT4 significantly improved grain yield (+359 kg ha−1), total grain N and NUE. VNT4 application improved growth during tillering and stem elongation (+10.7%), and N and 15N uptake between tillering and maturity (+7.3% N and +16.9% 15N) leading to a higher N accumulation at maturity (+9.3% N). This N mainly originated from fertilizer (+19.4% 15N) and was assimilated after the flag leaf stage in particular (+47.6% 15N). These effects could be related to maintenance of physiological functions of flag leaves as suggested by the enhancement of their nutrient status (especially S, Zn and Mo). The adoption of VNT4 as a UAN additive is an efficient agronomic practice to enhance wheat productivity under an oceanic temperate climate.