Background Physical exercise is a health promotion factor regulating gene expression and causing changes in phenotype, varying according to exercise type and intensity. Acute strenuous exercise in sedentary individuals appears to induce different transcriptional networks in response to stress caused by exercise. The objective of this research was to investigate the transcriptional profile of strenuous experimental exercise. Methodology RNA-Seq was performed with Rattus norvegicus soleus muscle, submitted to strenuous physical exercise on a treadmill with an initial velocity of 0.5 km/h and increments of 0.2 km/h at every 3 min until animal exhaustion. Twenty four hours post-physical exercise, RNA-seq protocols were performed with coverage of 30 million reads per sample, 100 pb read length, paired-end, with a list of counts totaling 12816 genes. Results Eighty differentially expressed genes (61 down-regulated and 19 up-regulated) were obtained. Reactome and KEGG database searches revealed the most significant pathways, for down-regulated gene set, were: PI3K-Akt signaling pathway, RAF-MAP kinase, P2Y receptors and Signaling by Erbb2. Results suggest PI3K-AKT pathway inactivation by Hbegf, Fgf1 and Fgr3 receptor regulation, leading to inhibition of cell proliferation and increased apoptosis. Cell signaling transcription networks were found in transcriptome. Results suggest some metabolic pathways which indicate the conditioning situation of strenuous exercise induced genes encoding apoptotic and autophagy factors, indicating cellular stress. Conclusion Down-regulated networks showed cell transduction and signaling pathways, with possible inhibition of cellular proliferation and cell degeneration. These findings reveal transitory and dynamic process in cell signaling transcription networks in skeletal muscle after acute strenuous exercise.

This work studied the effects of ethanol in the absence and presence of haloperidol under two experimental conditions. In protocol 1, rats were treated daily with ethanol (4 g/kg, p.o.) for 7 days, and received only haloperidol (1 mg/kg, i.p.) from the 8th day to the 14th day. In protocol 2, animals received ethanol, and the treatment continued with ethanol and haloperidol from the 8th day to the 14th day. Results show increases in alanine transaminase (ALT; 48% and 55%) and aspartate transaminase (AST; 32% and 22%) levels after ethanol or haloperidol (14 days) treatments, as compared with controls. Apolipoprotein A-1 (APO A1) levels were increased by haloperidol, after 7- (148%) but not after 14-day treatments, as compared with controls. Levels of lipoprotein (high-density lipoprotein (HDL-C)) tended to be increased only by ethanol treatment for 14 days. ALT (80%) and AST (43%) levels were increased in the haloperidol plus ethanol group (protocol 2), as compared with controls. However, an increase in APO A1 levels was observed in the haloperidol group pretreated with ethanol (protocol 1), as compared with controls and ethanol 7-day treatments. Triglyceride (TG) levels were increased in the combination of ethanol and haloperidol in protocol 1 (234%) and 2 (106%), as compared with controls. Except for a small decrease in haloperidol groups, with or without ethanol, as related to ethanol alone, no other effect was observed in HDL-C levels. In conclusion, we showed that haloperidol might be effective in moderating lipid alterations caused by chronic alcohol intake.

The diabetic foot is characterized by the loss of foot ulcerations and sensitivity. The use of orthopedic orthosis can prevent pathological changes in the diabetic foot. The objective of this study was to characterize materials used for producing orthopedic orthosis: silicone; pre-vulcanized latex of Hevea brasiliensis; Evapod, and Podadur. The Hevea brasiliensis latex material is widely indicated in the literature for biomedical purposes. Physical–mechanical properties were determined (properties of elastic deformation, resistance, durability, lightness, energy absorption, resistance to high temperatures, and chemical composition—ASTM International). In the tensile test, the latex reached 6.02 ± 0.33 MPa, having the best performance among the other materials. In the elastic module, the Podadur stood out, with 28.2 ± 0.89 MPa, compared to silicone with 0.42 ± 0.05 MPa. The most excellent Shore A hardness material was Podadur with 58%. As for the resilience, the Podadur presented a minimum value of 22%, while latex had 63%. Silicone was the densest material, with a density of 1.48 g/cm3, and Evapod and Podadur were the lightest, respectively, at 0.22 g/cm3 and 0.42 g/cm3. Morphologically, Evapod, Podadur, and latex presented open and interconnected cells, characteristics that gave them a more significant water absorption capacity. Silicone was the only material with no empty cells in its structure. In X-ray diffraction, Evapod, Podadur, and silicone materials presented well-defined crystallographic planes, whereas amorphous behavior characterized latex. Thermogravimetry showed weight loss between 240 and 650 °C in the four materials. In the fluorescence test, the presence of metals was observed in the composition of the four materials. Among the materials studied, the Podadur was the material that stood out, with some good properties for the development of orthopedic orthosis.

Aims: The present study aimed to stablish and characterize an optimized protocol conformation to obtain adequate RNA quality from rodents skeletal muscle samples for sequencing studies. Place and Duration of Study: The in vivo experiments and analyses were performed in the Laboratory of Biochemistry and Gene Expression – LABIEX of the Superior Institute of Biomedical Science – ISCB from the State University of Ceará - UECE. Between 2017-2020. Methodology: Were used 23 samples from male Wistar rat skeletal muscle, specifically from soleus muscle. Total RNA extraction was performed using the classic TRIzol® method and commercial kit, merging steps from both. Capillary electrophoresis in the Bioanalyzer platform was used for RNA quality evaluation. Results: (C) Analyzes of adapted protocol RNA concentration, RIN and rate 28S/18S showed satisfactory results. 28S/18S Ribosomal bands appear well defined, without small traces, which indicates RNA with high integrity and without contamination of genomic DNA. Conclusion: Obtained RNA quality and integrity data satisfied the exigencies for posterior RNA-seq.

Physical exercise (PE) in regularity is a well-characterized non-pharmaceutical intervention for good health and welfare. Molecular mechanisms regulated in response to PE can be scrutinized, with molecular biology, genomics, transcriptomics, and bioinformatics being inserted into exercise physiology studies. From a biotechnological perspective, omic datasets about physical exercise gene expression help identify phenotypic, genetic variance for different physical training phenotypes. Extensive lists of genes regulated by PE were dispersed within the literature, and the Fitnome Catalog (FitC) was created to reach some systematization of this information. Manual and online text-mining tools generated this dataset in PE human gene expression articles (2003-2014) with microarray, RNA-Seq, RT-PCR, and genotyping methods. Spreadsheets were developed with information on exercise protocol, experimental design, gender, age, number of individuals, analytical approach, gene ID, fold change and statistical data, and genetic architecture, encompassing 21 columns. The produced dataset (with 5,147 genes and 101,343 data points) provides experimental design, gene expression information, gene attributes, and references. Functional categorization of the FitC dataset and standardized information on PE-expressed genes were presented.