Coffee is the most appreciated beverage in worldwide; Brazil is the largest producer and exporter of this commodity. Organochlorine endosulfan was banned from the country in 2013 due to its teratogenic agent-related features. Since then, coffee plantations have experienced increased Hypothenemus hampei infestation rates. The aim of the current study is to assess variations in the rates of Coffea arabica fruits brocaded by H. hampei after the application of entomopathogenic fungal species Beauveria bassiana IBCB66. Experiments were carried out with 'Catuaí' and 'Mundo Novo' cultivars between 2018 and 2020, during the borer transit period. Three experiments were carried out based on the application of the aforementioned fungal species on the investigated coffee plant species, both by spraying and sprinkling, at 30-day intervals; 10 fruits were collected per face of each useful plant in each repetition. The experiment has followed a randomized blocks design with five treatments, including the control, and five repetitions, each. Beauveria bassiana Ecobass (IBCB66) wettable powder spray, at the concentration of 2 × 10 13 •ha -1 , was used in experiments I and II. On the other hand, the mix used in experiment III was prepared with blastospores at concentration of 5 × 10 12 •ha -1 blastospores + 0.1% Silwet. The sprinkling process in all three experiments has used dry aerial conidia at concentration of 2 × 10 13 •ha -1 . Collected data were subjected to analysis of variance (ANOVA), which was followed by Fisher's test at 5% probability level, in the SISVAR software. More than 35,000 fruits were assessed. In addition to variations between experiments, results have evidenced that the rate of brocaded fruits remained high.

O objetivo da pesquisa foi avaliar a eficiência de diferentes métodos de aplicação do fungo Beauveria bassiana IBCB66 no controle de Hypothenemus hampei. Foram realizados dez experimentos em laboratório com aplicação de suspensões com blastosporos e com conídios aéreos de B. bassiana IBCB66, por três métodos de aplicação: imersão, pulverização em Torre de Potter e caminhamento sobre conídios aéreos em pó. As suspensões foram padronizadas em 2,4x109 , 1x109 , 5x108 , 1x108 , 5x 107 e 1x107 conídios/mL-1 e o pó com concentrações 1,14x109 ; 5,7x109 ; 1,14x108 ; 5,7x107 ; 1,14x107 e 5,7x106 conídios/g. Em campo foram realizados experimentos com os métodos: sachês disseminadores; pulverização e polvilhamento na planta e em solo. Com os sachês foram dois experimentos por 24 horas; um experimento por 15 dias e um experimento por 30 dias. Para os experimentos de 24 horas e 15 dias, foram cinco tratamentos com sachês de diferentes materiais, com concentrações de conídios em arroz+fungo em 2x109 conídios/g e conídios aéreos em pó em 1x1010 conídios/g, com uma testemunha e cinco repetições. Para os sachês por 30 dias foram quatro tratamentos e uma testemunha, com a concentração do pó de conídios aéreos de B. bassiana em 5,98x109 conídios/g.

There are several studies that deal with the problem of infestation by Hypothenemus hampei in Coffeea arabica. However, the ways of applying products based on entomopathogenic fungi are the same as those used for chemicals. The objective of the research was to comparison different methods of application of Beauveria bassiana against H. hampei. The experiments were developed in laboratory, with blastospores and B. bassiana airborne conidia powders were carried out. Application methods based on immersion, Potter Spray Tower and walking on treated surface. Were three experiments were carried out for each method. Bioassays'design comprised seven treatments (including the control), with five repetitions (each) and 25 beetles per repetition. Suspensions were standardized at 2.4×10 9 , 1×10 9 , 5×10 8 , 1×10 8 , 5×10 7 and 1×10 7 conidia.mL -1 . Conidia powder presented initial concentration of 1.14×10 10 and it was standardized at 1.14×10 9 ; 5.7×10 9 ; 1.14×10 8 ; 5.7×10 7 ; 1.14×10 7 and 5.7×10 6 conidia.g -1 . A simultaneous test was also carried out based to the three application methods; treatment comprised conidia powder concentration of 1x10 8 and the control. Data were subjected to analysis of variance, which was followed by Tukey test (P<0.05) and, CL 50 , in the SPSS 12.1 software. The mortality better rates of H. hampei exposed to B. bassiana IBCB66 were1×10 8 (immersion), 1×10 9 (Potter Spray Tower) and 1×10 8 (walking), where as mean LC 50 were 6.1×10 8 , 4.1×10 8 , 2.4×10 8 conidia.mL -1 for each method, respectively. The highest mortality rate was recorded in the walking tests on the conidian powder at a concentration of 1×10 8 conidia.g -1 for the three repetitions of the method and also in the simultaneous test.