A high intramuscular fat content characterizes Wagyu (WY) cattle breed. Our objective was to compare beef from WY, WY-by-Angus, or Wangus (WN) steers with European, Angus-by-Charolais-Limousine crossbred steers (ACL), considering metabolic biomarkers pre-slaughtering and nutritional characteristics, including health-related indexes of the lipid fraction. The fattening system with olein-rich diets and no exercise restriction included 82 steers, 24 WY, 29 WN, and 29 ACL. The slaughter ages and weights were (median and interquartile range) 38.4 mo.-old (34.9–40.3 mo.) and 840 kg (785–895 kg) for WY; for WN, 30.6 mo. (26.9–36.5 mo.) and 832 kg (802–875 kg), and for ACL steers, 20.3 mo.-old (19.0–22.7 mo.) and 780 kg (715–852 kg). Blood lipid-related metabolites, except for non-esterified fatty acids (NEFA) and low-density level cholesterol (LDL), were higher in WY and WN than in ACL, while glucose was lower in WY and WN. Leptin was higher in WN than in ACL. Pre-slaughtering values of plasma HDL underscored as a possible metabolic biomarker directly related to beef quality. The amino-acid content in beef did not differ among experimental groups, except for more crude protein in ACL. Compared to ACL, WY steers showed higher intramuscular fat in sirloin (51.5 vs. 21.9%) and entrecote (59.6 vs. 27.6%), more unsaturated fatty acids in entrecote (55.8 vs. 53.0%), and more oleic acid in sirloin (46 vs. 41.3%) and entrecote (47.5 vs. 43.3%). Compared to ACL entrecote, WY and WN showed better atherogenic (0.6 and 0.55 vs. 0.69), thrombogenicity (0.82 and 0.92 vs. 1.1), and hypocholesterolemic/hypercholesterolemic index (1.9 and 2.1 vs. 1.7). Therefore, beef’s nutritional characteristics depend on breed/crossbred, slaughtering age and cut, with WY and WN entrecote samples showing a healthier lipid fraction.

We report an outbreak of pharyngeal inflammation in an extensive limousine herd, in central Spain, associated with a novel genotype (ST63) of Pasteurella multocida capsular serogroup B (capB). The outbreak affected cattle of all ages, reaching a herd‐level death rate of 17.6% and case fatality rate of 93.3%. Affected animals showed throat inflammation, respiratory distress and finally death, without characteristics of depression or haemorrhagic septicaemia in an acute course. Postmortem examination only revealed inflammation, including oedema in the pharynx and surrounding tissues. None of the necropsied animals was septicaemic and none showed multisystem petechial haemorrhages, pneumonia or enteritis characteristics of haemorrhagic septicaemia. P. multocida was isolated, serotyped and characterised as capB and, with the multihost P. multocida multilocus sequence typing database, isolates were assigned to the new sequence type ST63. Results of this study suggest that capB P. multocida type ST63 should be considered in the differential diagnoses of acute throat inflammation in cattle.