A B S T R A C TThe fungistatic activity, especially during storage, can lead to rapid deterioration in the nutritional quality of grains, and reduce their use and disposal in industrial process due to contamination with toxins (aflatoxins and others). Among the technologies identified as promising in controlling these microorganisms, there is ozonation. The objective of this study was to determine the concentration and the saturation time of ozone gas in rice grains and set the effective ozonation disinfection time in filamentous fungi and yeast. Rice grains (14.3% w.b.) were inoculated with Penicillium spp. and Aspergillus spp. and, subsequently, ozonized at the concentration of 10.13 mg L -1 , under continuous flow of 1.0 L min -1 , in five periods of exposure (12, 24, 36, 48 and 60 h). Ozone gas concentration and saturation time in rice grains were 5.00 mg L -1 and 13.97 min, respectively. There was a reduction of 3.8 log cycles (100%) in the count of yeasts and complete inhibition of fungal from the genera Aspergillus and Penicillium in ozonized grains.Ozônio como agente fungicida em grãos de arroz R E S U M OA atividade fungiostática pode, principalmente durante o armazenamento, levar à rápida deterioração na qualidade nutricional dos grãos e reduzir seu aproveitamento e inutilização em processo industrial pela contaminação com toxinas (aflatoxinas entre outras). Dentre as tecnologias apontadas como promissoras no controle desses microrganismos se destaca a ozonização. Objetivou-se, com este estudo, determinar a concentração e o tempo de saturação do gás ozônio em grãos de arroz e definir o tempo de ozonização eficaz na desinfecção de fungos e leveduras. Grãos de arroz (14,3% b.u.) foram inoculados com Penicillium spp. e Aspergillus spp. e posteriormente sua ozonização foi realizada na concentração de 10,13 mg L -1 , em fluxo contínuo de 1,0 L min -1 , em cinco períodos de exposição (12, 24, 36, 48 e 60 h). A concentração e o tempo de saturação do gás ozônio nos grãos de arroz foram de 5 mg L -1 e 13,97 min, respectivamente. Observou-se redução em 3,8 ciclos log (100%) na contagem de leveduras e completa inibição para os fungos dos gêneros Aspergillus e Penicillium nos grãos ozonizados.
Due to the emergence of large microalgae farms and increased competition in this sector, the search for higher productivity is common. One way to achieve this goal in microalgae production is to optimize the factors that influence their growth during the cultivation stage to increase the accumulation of bio-compounds of interest. In this stage, the factors that most influence are: nutrition, gas diffusion, light intensity and quality and, finally, stirring, which directly affects all other factors. Thus, a review and an evaluation of the influence and importance of stirring were performed in the present study. The nutrients that most influence biomass accumulation are carbon, nitrogen and phosphorus, but their proportion is directly related to the proposed objective for microalgae. In the diffusion of gases, it is essential to supply adequate CO2 for the growth of microalgae, and flue gases can be used. Also, it is necessary to ensure proper removal of photosynthetic O2, which could inhibit microalgae metabolism and slow their growth rate. It is important to provide the appropriate light intensity for photosynthesis, but excess may cause photoinhibition in cultivation. Stirring is of paramount importance to ensure nutrient distribution in the medium, gas diffusion (incorporation of CO2 and removal of O2) and adequate exposure of microalgae to light, reducing the effects of photoinhibition and self-shading.
Orbital myositis in systemic lupus erythematosus (SLE) is a rare entity with risk of serious complications. Timely treatment with effective immunosuppressors is desirable. We report a case of a 32-year-old female patient with SLE who presented with an acute ocular pain and extraorbital muscle thickening, consistent with orbital myositis. Association with SLE was made after exclusion of other aetiologies. Due to refractoriness to steroids, off-label rituximab was initiated with clinical and imaging parameter improvement.
The objective of this work was to evaluate the biomass burning process in a furnace, using chip as a raw material and an oxygen sensor (lambda probe) to monitor the percentage of oxygen in the gases exhausted during combustion, aiming to maintain the percentage of the coefficient of excess air (α) in the operating range of 1.20 to 1.25%, considered ideal for the biomass (chip) used in the study. Once the excess air is identified at the upper furnace exit, the air entrance in the system will be closed by means of a butterfly valve using a servo motor driven by a Programmable Logic Controller (PLC). The valve was opened or closed according to the oxygen level; when it was lower than 1.20%, the door remained open, and it was closed when the level reached 1.25%, finishing the cycle of the process. The open and closed states of the valve occurred by means of two reed switch magnetic key sensors installed in the air intake system. For the control, monitoring and data acquisition, a supervisory system created using the Elipse SCADA software was used so it was possible to obtain a system database, which will provide important information to maximize the efficiency of the furnace.
ResumoModificações no teor de vários componentes da secreção pancreática e alterações histopatológicas desse órgão são verificadas após injeção de veneno do escorpião Tityus serrulatus em ratos. Entre essas alterações, podem ser citadas pancreatite aguda e crônica após uma única injeção de veneno. Os achados compatíveis com pancreatite aguda foram encontrados a partir de 10 minutos após a intoxicação e, 20 dias após, a maioria dos animais exibiu achados histológicos típicos de pancreatite crônica. O objetivo do presente trabalho foi determinar a cinética das alterações pancreáticas que ocorrem ao longo do tempo, após injeção endovenosa de veneno bruto de escorpião em ratos. Os animais, após jejum de 24-48 horas, receberam uma injeção venosa, pela veia peniana, de 0,2 ml de uma solução de cloreto de sódio (grupo controle) ou de 0,2 ml de uma solução contendo 300 mg/ml de veneno bruto do escorpião Tityus serrulatus (60 mg/animal). Os animais foram sacrificados em grupos de 5 a 10 cada, aos 10, 20 e 40 minutos e 1, 2, 3, 4, 6, 8, 10, 12, 14, 16, 18 e 20 dias após a injeção. O pâncreas foi retirado e processado para estudo histológico. Pancreatite aguda (PA) foi observada nos grupos sacrificados após 10, 20 e 40 minutos, 24 e 48 horas após a injeção. Achados compatíveis com pancreatite crônica (PC) foram detectados nos animais sacrificados entre 4 a 20 dias após a injeção. No grupo de animais sacrificados 3 dias após, observaram-se, no mesmo animal e, às vezes, no mesmo corte histológico, alterações características de PA (degranulação, vacuolização acinar, infiltração difusa de linfócitos e lesões granulomatosas) e, surpreendentemente, de PC (dilatação ductal com rolhas protéicas, hiperplasia ductal e hiperplasia dos linfonodos peripancreáticos), caracterizando a fase de transição da pancreatite aguda para pancreatite crônica. Todos os grupos apresentaram mobilização e degranulação de mastócitos, sugerindo a participação dessas células na etiopatogênese dessas lesões. Em 50% dos animais dos grupos sacrificados aos 18 e 20 dias após o veneno foram também observados, ao lado das lesões inflamatórias, hiperplasia das ilhotas de Langerhans. Palavras-chavePancreatite aguda, pancreatite crônica, secreção pancreática, veneno de escorpião
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