The present study was aimed to assess the diversity, distribution and abundance of macrozoobenthos in Parbati River (Madhya Pradesh). During the present investigation, 5 sampling stations were selected to collect the samples. Shannon diversity index and Margalef's richness index was used on benthic data obtained during the survey. A total of 50 taxa of macrobenthic fauna were recorded from different sampling stations of Parbati River. The phylum Arthropoda was found dominant followed by mollusca and annelida. The maximum diversity and richness were recorded during winter season while minimum diversity was recorded during monsoon. Among the EPT, Ephemeroptera were reported only at the reference site, while Plecoptera and Trichoptera were absent at all the stations.