Background: Irritable bowel syndrome (IBS) is a common chronic gastrointestinal disease identified by frequent episodes of abdominal pain, bloating and disturbance of bowel habits which are owed to several factors including environmental, stressor, and certain food and medications. This study aimed to estimate the prevalence of IBS among Princess Nourah bint Abdulrahman University (PNU) students in health and non-health colleges, and to determine its associated factors and impact. Methods: A cross-sectional study was conducted among 514 female students of PNU's health and non-health colleges. Participants were selected by simple random sampling. A self-administered questionnaire was used to collect data including sociodemographic data, Rome IV scale, in addition to risk factors and impact related variables. Results: The prevalence of IBS among PNU students was 31.9%. Health students had higher prevalence as compared to non-health students (36.6% vs 27.2%). Emotional stress and traumatic life events were more commonly reported among students with IBS (p