Crude oil (petroleum) is a naturally occurring complex composed of hydrocarbon deposits and other organic materials. Bioremediation of crude oil-polluted sites is restricted by the biodiversity of indigenous microflora. They possess complementary substrates required for degrading the different hydrocarbons. In the current study, four yeast strains were isolated from different oil reservoirs in Riyadh, Saudi Arabia. The oil-biodegradation ability of these isolates showed variable oxidation effects on multiple hydrocarbons. The scanning electron microscopy (SEM) images showed morphological changes in Candida isolates compared to the original structures. The drop-collapse and oil emulsification assays showed that yeast strains affected the physical properties of tested hydrocarbons. The content of biosurfactants produced by isolated strains was quantified in the presence of different hydrocarbons to confirm the oil displacement activity. The recovery assays included acid precipitation, solvent extraction, ammonium sulfate, and zinc sulfate precipitation methods. All these methods revealed that the amount of biosurfactants correlates to the type of tested hydrocarbons, where the highest amount was produced in crude oil contaminated samples. In conclusion, the study highlights the importance of Candida isolated from contaminated soils for bioremediation of petroleum oil pollution. That raises the need for further analyses on the microbes/hydrocarbon degradation dynamics.

Currently, the bioremediation of petroleum hydrocarbons employs microbial biosurfactants because of their public acceptability, biological safety, and low cost. These organisms can degrade or detoxify organic-contaminated areas, such as marine ecosystems. The current study aimed to test the oil-biodegradation ability of the fungus Drechslera spicifera, which was isolated from contaminated soil samples in Riyadh, Saudi Arabia. We used hydrocarbon tolerance, scanning electron microscopy, DCPIP, drop-collapse, emulsification activity, recovery of biosurfactants, and germination assays to assess the biodegradation characteristics of the D. spicifera against kerosene, crude, diesel, used, and mixed oils. The results of DCPIP show that the highest oxidation (0.736 a.u.) was induced by crude oil on the 15th day. In contrast, kerosene and used oil had the highest measurements in emulsification activity and drop-collapse assays, respectively. Meanwhile, crude and used oils produced the highest amounts of biosurfactants through acid precipitation and solvent extraction assays. Furthermore, the biosurfactants stimulated the germination of tomato seeds by more than 50% compared to the control. These findings highlight the biodegradation ability of D. spicifera, which has been proven in the use of petroleum oils as the sole source of carbon. That might encourage further research to demonstrate its application in the cleaning of large, contaminated areas.

Background: Microbial biodegradation of oil-hydrocarbons is one of the sustainable and cost-effective methods to remove petroleum spills from contaminated environments. The current study aimed to investigate the biodegradation abilities of three Fusarium isolates from oil reservoirs in Saudi Arabia. The novelty of the current work is that the biodegradation ability of these isolates was never tested against some natural hydrocarbons of variable compositions, such as Crude oil, and those of known components such as kerosene and diesel oils. Methods: The isolates were treated with five selected hydrocarbons. The hydrocarbon tolerance test in solid and liquid media was performed. The scanning electron microscope (SEM) investigated the morphological changes of treated fungi. 2, 6-Dichlorophenol Indophenol (DCPIP), drop collapse, emulsification activity, and oil Spreading assays investigated the biodegradation ability. The amount of produced biosurfactants was measured, and their safety profile was estimated by the germination assay of tomato seeds. Results: The tolerance test showed enhanced fungal growth of all isolates, whereas the highest dose inhibition response (DIR) was 77% for Fusarium proliferatum treated with the used oil (p < 0.05). SEM showed morphological changes in all isolates. DCPIP results showed that used oil had the highest biodegradation by Fusarium verticillioides and Fusarium oxysporum. Mixed oil induced the highest effect in oil spreading, drop collapse, and emulsification assay caused by F. proliferatum. The highest recovery of biosurfactants was obtained by the solvent extraction method for F. verticillioides (4.6 g/l), F. proliferatum (4.22 g/l), and F. oxysporum (3.73 g/l). The biosurfactants produced by the three isolates stimulated tomato seeds’ germination more than in control experiments. Conclusion: The current study suggested the possible oil-biodegradation activities induced by three Fusarium isolates from Riyadh, Saudi Arabia. The produced biosurfactants are not toxic against tomato seed germination, emphasizing their environmental sustainability. Further studies are required to investigate the mechanism of biodegradation activities and the chemical composition of the biosurfactants produced by these species.

Wheat is one of the most important sources of food worldwide. A selection index helps in making selection decisions and permits the exploitation of information on correlated traits to improve yields. Two cycles of pedigree selection based on the desired-genetic-gain selection index were imposed to identify the best index to isolate promising lines. The base population was composed of 120 families of bread wheat in the F6 generation. Eight combinations were constructed from six traits, i.e., days to heading (DH), number of spikes/plant (NS/P), grain yield/plant (GY/P), number of grains/spike (NG/S), mean spike weight (MSW) and mean grain weight (GW). The narrow-sense heritability of NS/P, NG/S, MSW and GW increased from cycle 1 to cycle 2, revealing an increase in the observed gain and homogeneity of the selected families for these traits from cycle to cycle. After the second cycle, the observed gain in GY/P ranged from 9.5 to 23.75% of the mid-parent. The best index for improving GY/P was index 2 (composed of GY/P, NS/P, NG/S, MSW and GW). The indices involving DH were inferior for improving GY/P. The desired-genetic-gain index was efficient in simultaneously improving several involved traits and was a good method to preserve genetic variability. Furthermore, six superior promising families were identified.

Crude oil (petroleum) is a naturally occurring complex composed of hydrocarbon deposits and other organic materials. Bioremediation of crude oil-polluted sites is restricted by the biodiversity of indigenous microflora. They possess complementary substrates required for degrading the different hydrocarbons. In the current study, four yeast strains were isolated from different oil reservoirs in Riyadh, Saudi Arabia. The oil-biodegradation ability of these isolates showed variable oxidation effects on multiple hydrocarbons. The scanning electron microscopy (SEM) images showed morphological changes of Candida isolates compared to the original structures. The drop-collapse and oil emulsification assays showed that yeast strains affected the physical properties of tested hydrocarbons. The content of biosurfactants produced by isolated strains was quantified in the presence of different hydrocarbons to confirm the oil displacement activity. The recovery assays included acid precipitation, solvent extraction, ammonium sulfate, and zinc sulfate precipitation methods. All these methods revealed that the amount of biosurfactants correlates to the type of tested hydrocarbons, where the highest amount was produced in crude oil contaminated samples. In conclusion, the study highlights the importance of Candida isolated from contaminated soils for bioremediation of petroleum oil pollution. That raises the need for further analyses on the microbes/hydrocarbon degradation dynamics.