This study was done to find out the effect of seasonal variations on the hematological and blood biochemical parameters of local cattle raised in Maysan Governorate. A number of 40 healthy cows were selected for the study during winter and summer seasons, blood samples were collected in each of the two seasons and were analyzed for some hematological and biochemical parameters. The results in the winter season showed significantly higher values of Mean Corpuscular Volume, glucose, total protein, albumin, globulin, bilirubin and creatinine but lower values of total white blood cells count, differential leukocyte count, hemoglobin concentration, Mean Corpuscular Hemoglobin, Mean Corpuscular Hemoglobin Concentration, AST and ALT than that of the same animals during the summer season. However, no significant differences in the erythrocyte count, haematocrite between the two seasons were observed in the local cattle.