Enamel fluid in human teeth plays an important role in the permeability and mechanical property of the enamel. It can be invetigated as fluid droplets at the enamel surface by using the replica technique. The experiments were done on 17 premolars of 10 subjects (aged 10–25 yrs) to be extracted during orthodontic treatment. Teeth were divided into 2 groups. In the first group (
n
= 11), the fluid accumulated on the mid buccal of the enamel surface was recorded with the impression material both
in vivo
and
in vitro. In vitro
replicas were obtained when the pressure in the pulp cavity held at 0, 20, 100, and 200 mmHg above atmospheric. They were examined by a scanning electron microscope. For the other group (
n
= 10), each tooth was prepared for fluid flow measurement during which the same set of pulpal pressures was applied as mentioned in the other study
[1]
. However, four teeth of 3 subjects were arranged for the recording of fluid conductance, while the replica impression at the mid buccal surface was also taken simultaneously under each of the applied pulpal pressure. This dataset describes subjects’ baseline characteristics, including their corresponding records of the droplets’ observations both
in vivo
and
in vitro
. Also, data of
in vitro
fluid flow measurements were detailed according to the applied pulpal pressure. The patterns of changes of the fluid flow rates and the droplets’ numbers provided in this dataset can be used to validate tests of agents affecting the structure and permeability of the enamel.
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