In the present study, 100 human, dry adult sterna, 45 male and 55 female, from various medical colleges of Saurashtra region of Gujarat were measured for length of manubrium, length of mesosternum and combined length of manubrium and mesosternum. The data was statistically analysed for mean, standard deviation and p value. “The Rule of 136” given by Ashley (1956) was applied to determine the sex of sterna and to determine the number of male and female sterna obeying the rule. Statistically significant difference in mean values of length of manubrium, length of mesosternum and combined length of both sexes was observed. “Rule of 136” determined 34 male and 66 female sterna, while 71.11% (32) of male sterna and 96.34% (53) of female sterna obeyed the rule. It concluded that of all the three parameters measured, combined length of manubrium and mesosternum was the best discriminating parameter of sex and Rule of 136 was helpful in determination of sex