The hepatoprotective and antioxidant activity of methanolic extract of whole plant of Gardenia Gummifera (GGME) was evaluated against paracetamol induced liver damage in rats. And the GGME fractionated based on polarity of solvents with toluene, ethanol, 2-butanone, n-butanol and petroleum ether. The substantially elevated serum enzymatic levels of Aspartate Aminotransferas (AST), Alanine Transaminase (ALT), Alkaline Phosphate (ALP) and total bilirubin were restored towards normalization significantly by the GGME in a dose dependent manner in paracetamol induced liver damage. The biochemical observations were supplemented with histopathological examination liver sections high protection against paracetamol induced hepatoxicity. Further investigation continued with GGME fractions, 2-Butanone Fraction (BTF) and nbutanol Fraction (BAF) substantially elevated serum enzymatic levels of AST, ALT, ALP and Total Bilirubin (TB) were restored towards normalization significantly The significant values showed with n-butanol fraction on pentobarbitone induced sleeping time in mice and the liver weight of paracetamol induced liver damaged in rats. Meanwhile, in vitro antioxidant activities such as DPPH scavenging assay was also screened which were also found significantly positive in a dose dependent manner. The results of this study strongly indicate that GGME and n-butanol fraction results potent hepatoprotective activity against paracetamol induced liver damage in experimental animals.