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Fusarium oxysporium f. sp. cubense (Foc) race is an archetypally soil-born fungus causing chief fiscal damage to farmers growing banana plants. Supervising Foc is attained by the habit of chemical fungicides which undesirably harm the soil fertility. Our investigations intended to activate the aptitude of Trichoderma strains for activation of PGPR and IIR. The activation and accretion of defence enzymes such as Polyphenol Oxidase (PPO), Phenylalanine Ammonia-Lyase (PAL) and Peroxidase (PO) are been amplified by inoculation, and treatment with a combination of Trichoderma harzanium and Trichoderma viride strains (T3-H1+V1). Besides combination-based inoculum treatments gave best results than individual and fungicide-treated plantlets for supervising Foc pathogenesis. Based on the results we conclude that usage of T1-H1 and T2-H2 treatments with a combination such as T3-H1+V1 gave promising results and can be used as a prominent biocontrol formulation for inducing defence enzymes and PGPR in Musa paradisica cv. Malnad Rasbale.

Objective: The present study was designed to evaluate the chemical composition of the essential oil of Buchanania lanzan Spreng extracted from the seeds and to evaluate in vitro antimicrobial antioxidants and molecular docking studies of the major bioactive compounds of essential oil. Methods: The essential oil was obtained by hydrodistillation of the B. lanzan seeds and analyzed by gas chromatography-mass spectrometry (GC-MS). Antibacterial activity was evaluated against Pseudomonas aeruginosa, Salmonella typhi, Vibrio cholerae, Staphylococcus aureus, and Streptococcus pneumoniae clinical isolates by disk diffusion method and resazurin assay determined the minimum inhibitory concentration. The in vitro antioxidant activity was determined by 2,2-diphenyl-1-picrylhydrazyl (DPPH) and hydrogen peroxide (H2O2) scavenging assay; the essential oil major bioactive compounds are Androstan-3-ol, Campesterol, and γ-Sitosterol were docked against bacterial protein DNA gyrase. Results: GC-MS analysis exhibited the presence of 19 bioactive compounds. The essential oil showed that significant antibacterial activity was noticed against V. cholerae and S. typhi with the highest zone of inhibition 15.67–1.20 and 13.83–0.33, respectively. Antioxidant activity in DPPH and H2O2 scavenging assays with IC50 values of 134.23 and 191.24, respectively. The molecular docking of Androstan-3-ol and γ-Sitosterol with bacterial DNA gyrase unveiled a good binding affinity of −6.4 kcal/mol and −6.3 kcal/ mol, respectively. Conclusion: It could be concluded that the essential oils potential sources of antibacterial, antioxidant activities, and molecular docking of bioactive components. The results of this study provide partial scientific support for the traditional application of essential oils to cure diarrhea and also major bioactive compounds responsible for important biological activities.