The purpose of the present study was to formulate Solid Lipid Nanoparticles (SLNs) of Ganciclovir (GCV) in combination with Chitosan and Piperine for absorption enhancement effect. GCV loaded SLNs were prepared by hot homogenization method, optimized and characterized. Formulated SLNs were incorporated with absorption enhancers and characterized for invitro absorption (with chicken intestine), histopathological and invivo pharmacokinetic studies. Invitro absorption studies revealed that the permeability coefficient of the prepared formulation is more when compared to the pure drug, so the permeability is more for prepared formulation. In vivo pharmacokinetic study showed a significant increase in the Cmax, AUC, biological half-life and decrease in elimination rate constant for prepared formulation compared to pure drug. Histopathological studies also showed mild reversible damage of epithelial cells with Chitosan which indicates the safety and efficacy of the formulation. Thus, GCV loaded SLNs prepared with Chitosan can be clinically promising for enhancing the oral, intestinal absorption of the said BCS Class-III drug.

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The present experiment was conducted in Factorial Completely Randomized Design with three factors at unequal levels and replicated thrice. At 120 days second order interactions between media, IBA concentration and method of application (M×C×A), maximum fresh weight of shoot was (12.45 g) recorded with M1C1A2. While, minimum fresh weight of shoot (6.06g) observed in M1C3A1. Highest dry weight of shoot (4.22g) was exhibited by M1C1A2 which was on par with M1C2A2 (4.05 g) while lowest dry weight of shoot was in M2C2A2 (1.56 g). Maximum root and shoot ratio (0.23) was with M1C1A2 followed by M3C1A2 (0.19) while minimum root and shoot ratio (0.07) recorded in M1C2A1.