Nephrops norvegicus (L.) from fishing grounds on the west coast of Scotland has been found to harbour infection by a species of parasitic dinoflagellate. Chromosome morphology and ultrastructural features suggest that the parasite is a member of the botanical order Syndiniales, possibly related to Hematodinium perezi Chatton & Poisson 1931. Cells invading the haemal spaces, however, show no signs of flagella. Mode of transmission IS not yet known, and a flagellate spore stage has not been identified. Infection appears to be fatal to its host, the main cause of death possibly being disruption of gas transport and tissue anoxia caused by the proliferation of large numbers of dinoflagellate cells in the haemolymph. Severe infection has an adverse effect on meat quality that has provoked comment from fisherman and processors. Affected lobsters have been found at all west coast sites surveyed, with peak infection rates reaching 70 % of trawled samples. Infection occurrence shows marked seasonality coincident with the annual moult perlod of N. norvegicus. An increased prevalence of infection has been observed recently in some areas.

How we manage farming and food systems to meet rising demand is pivotal to the future of biodiversity. Extensive field data suggest impacts on wild populations would be greatly reduced through boosting yields on existing farmland so as to spare remaining natural habitats. High-yield farming raises other concerns because expressed per unit area it can generate high levels of externalities such as greenhouse gas (GHG) emissions and nutrient losses. However, such metrics underestimate the overall impacts of lower-yield systems, so here we develop a framework that instead compares externality and land costs per unit production. Applying this to diverse datasets describing the externalities of four major farm sectors reveals that, rather than involving tradeoffs, the externality and land costs of alternative production systems can co-vary positively: per

Greenhouse gas emissions from global agriculture are increasing at around 1% perannum, yet substantial cuts in emissions are needed across all sectors 1 . The challenge of reducing agricultural emissions is particularly acute, because the reductions achievable by changing farming practices are limited 2,3 and are hampered by rapidly rising food demand 4,5 . Here we assess the technical mitigation potential offered by land sparingincreasing agricultural yields, reducing farmland area and actively restoring natural habitats on the land spared 6 . Restored habitats can sequester carbon and can offset emissions from agriculture. Using the United Kingdom as an example, we estimate net emissions in 2050 under a range of future agricultural scenarios. We find that a landsparing strategy has the technical potential to achieve significant reductions in net emissions from agriculture and land-use change. Coupling land sparing with demandside strategies to reduce meat consumption and food waste can further increase the technical mitigation potential, however economic and implementation considerations might limit the degree to which this technical potential could be realised in practice.We projected the mitigation potential of land sparing in the United Kingdom with reference to its binding commitment to reduce emissions by 80% by 2050 (relative to 1990 levels) 7 . We began by identifying a technically plausible range in the future yields of all major crop and livestock commodities produced in the UK, based on historic trends and future potential. We define yields as the annual tonnage of production per hectare for crops and the feed conversion ratio (feed consumed per kilogram of production) for livestock. Future yields could vary across a wide range, driven by a number of biophysical, technical and socioeconomic factors [8][9][10][11] . We assessed the likely bounds of this range based on an assessment of technical potential and reflect this in our projections, which span yield declines through to sustained long-term growth averaging 1.3% per annum across all commodities 3 (Table 1; Supplementary Fig. 1; Supplementary Discussion). For the avoidance of doubt, we do not equate our lower yielding scenarios with 'land sharing'.We next projected emissions attributable to UK agricultural production out to 2050, quantifying all sources of emissions that would be affected by a land-sparing strategy. We therefore quantified not only emissions reported under 'Agriculture' in the UK's greenhouse gas inventory 12 , but also emissions related to agriculture but reported in other sectors (e.g. farm energy use, agro-chemical production and land-use change), and emissions arising overseas due to imported feed for livestock (see Supplementary Table 2 for all emissions sources quantified). Our projections assumed that agricultural production increases from present levels in proportion to projected demand growth (Supplementary Table 1). In certain scenarios, projected UK farming capacity does not keep pace with demand growth. In such cases ...

Chinese hamster ovary (CHO) cell lines are frequently used as hosts for the production of recombinant therapeutics, such as monoclonal antibodies, due to their ability to perform correct post-translational modifications. A potential issue when utilizing CHO cells for therapeutic protein production is the selection of cell lines that do not retain stable protein expression during long-term culture (LTC). Instability of expression impairs process yields, effective usage of time and money, and regulatory approval for the desired therapeutic. In this study, we investigated a model unstable GS-CHO cell line over a continuous period of approximately 100 generations to determine markers of mechanisms that underlie instability. In this cell line, stability of expression was retained for 40-50 generations after which time a 40% loss in antibody production was detected. The instability observed within the cell line was not due to a loss in recombinant gene copy number or decreased expression of mRNA encoding for recombinant antibody H or L chain, but was associated with lower cumulative cell time values and an apparent increased sensitivity to cellular stress (exemplified by increased mRNA expression of the stress-inducible gene GADD153). Changes were also noted in cellular metabolism during LTC (alterations to extracellular alanine accumulation, and enhanced rates of glucose and lactate utilization, during the exponential and decline phase of batch culture, respectively). Our data indicates the breadth of changes that may occur to recombinant CHO cells during LTC ranging from instability of recombinant target production at a post-mRNA level to metabolic events. Definition of the mechanisms, regulatory events, and linkages underpinning cellular phenotype changes require further detailed analysis at a molecular level.

An efficient rapid protein expression system is crucial to support early drug development. Transient gene expression is an effective route, and to facilitate the use of the same host cells as for subsequent stable cell line development, we have created a high-yielding Chinese hamster ovary (CHO) transient expression system. Suspension-adapted CHO-K1 host cells were engineered to express the gene encoding Epstein-Barr virus (EBV) nuclear antigen-1 (EBNA-1) with and without the coexpression of the gene for glutamine synthetase (GS). Analysis of the transfectants indicated that coexpression of EBNA-1 and GS enhanced transient expression of a recombinant antibody from a plasmid carrying an OriP DNA element compared to EBNA-1-only transfectants. This was confirmed with the retransfection of an EBNA-1-only cell line with a GS gene. The retransfected cell lines showed an increase in transient expression when compared with that of the EBNA-1-only parent. The transient expression process for the best CHO transient cell line was further developed to enhance protein expression and improve scalability by optimizing the transfection conditions and the cell culture process. This resulted in a scalable CHO transient expression system that is capable of expressing 2 g/L of recombinant proteins such as antibodies. This system can now rapidly provide gram amounts of recombinant antibody to supply preclinical development studies that has comparable product quality to antibody produced from a stably transfected CHO cell line.