Chronic kidney disease (CKD) is a growing international health concern with elevating mortality rate in the world today and it is generally a progressive and irreversible issue. It is defined by changes in kidney shape, function, or both lasting at least three months. Renal disease is commonly associated with Abnormalities of minerals and electrolyte. Levels of Ca change and the FGF23 increase early in CKD. This study was designed to show the Correlation between FGF23 and Ca in chronic kidney disease patients. One hundred blood samples (57males and 43 females) from CKD patients have been gathered from Al-Yarmouk Teaching Hospital, Baghdad Teaching Hospital and Al-Karama Teaching Hospital during the period from December 2021 to March 2022 besides 50 samples as apparently healthy control. The age range in the current study was at 33-75 years for CKD patient and 30-65 years for control. The fibroblast growth factor 23 was screened by an ELISA method and serum Calcium, by cobas c111 analyze. Ca, FGF23 is statistically highly significant at p-value =0.001.  The receiver operation characteristic (ROC) curve results revealed that the sensitivity and specificity for FGF23 was 100%. The conclusion of the current study shows that the. FGF 23 was high in all CKD patients and Ca was high in two patients, normal in thirty-seven patients, and decreased in sixty-one patients.

An over all of thirty females experiencing idiopathic POF and thirty controls have been studied and among them only six patients and four control were sent to sequence. The AMH gene fragments AMH1, AMH2 and AMH5 generates 530 bp, 288 bp and 1080 bp respectively were amplified. Fragments were sequenced and the results show six out of eight observed SNPs was appeared missense mutations rs764149385, rs753970896, rs10407022, rs61736575, rs1438510393 and rs950459635 Whereas, the novelty of two discovered SNPs were validated, including g.2189550T>G and g.2189667T>G. the novel two SNPs has found to cause missense effect of V73G and L112W, respectively present in AMH1 fragment. While AMH2 fragments how two SNPs, including rs17854573 and rs8112524, in AMH5 fragment, five SNPs were observed, including rs1254589021, rs1478367998, rs7252789 and rs10417628, while only one SNP (g.7156-7157insC) was proven to be novel insertion-deletion SNP, synonymos and missense mutation in POF patient. Our results indicated that the POF Iraqi patients has genetic variant in AMH gene which participate in POF pathophysiology.