A behavior-based approach to quality has been proposed to highlight the impact that upstream behaviors have on the overall outcomes of construction projects. The focus of this pioneering approach is first to understand that certain behaviors lead to conversations in which expectations are clearly identified and understood by the different project participants, and then to set measurable acceptance criteria so that the final result can be compared with what was agreed. Previous research has described the approach and provided positive results in satisfying client's expectations, but the process to achieve such outcomes has not been captured. This paper captures the implementation of this behavior-based quality (BBQ) approach to quality management, that has as its main goal to have no surprises, zero rework, and to improve delivery of value to all the project participants engaged at any point of a construction project. Construction projects are to be planned first for quality to fully understand expectations of what the team should build, then for safety to identify any potential risks associated with the processes to build the agreed work and define how tasks will be built in a safe manner, and then for production to secure flow and an adequate use of resources.

Shigella spp. are the causative agent of bacillary dysentery, a major cause of food-borne morbidity and mortality worldwide. These organisms are recently evolved, polyphyletic pathovar of E. coli, and since their divergence they have undergone multiple cases of gene gain and gene loss and understanding how gene inactivation events alter bacterial behaviour represents an important objective to be better able to understand how virulence and other phenotypes are affected. Here, we identify a frameshift mutation in the pmrD gene of S. flexneri that although it would be predicted to make a functional, full-length protein, no such production occurs, likely due to the non-optimal spacing between the translational initiation site and the Shine-Dalgarno sequence. We show that this loss severs the normal connection between the PhoPQ two-component regulatory system and the PmrAB two-component regulatory system, abrogating low Mg2+ mediated cationic antimicrobial peptide and polymyxin B resistance, while maintaining normal PmrAB-mediated polymyxin B resistance. In contrast, S. sonnei maintains a functional PmrD protein and canonical signaling through this regulatory network. This species specific gene loss suggests that S. flexneri and S. sonnei have evolved different regulatory responses to changing environmental conditions.