Background: Vitamin D deficiency or insufficiency is very prevalent, but it is most common in pregnant women and infants, where it is related to a wide range of unfavorable consequences, for example, preeclampsia and premature birth. This study aims to: (1) Evaluate the negative implications of low levels of vitamin D on neonates; (2) Analyze the prevalence of Vitamin-D insufficiency/deficiency amongst mothers and their newborns, as well as the features associated with it. Patients and methods: Between July 2 and October 31, 2021, researchers at Baghdad's "Al-Elwiya teaching hospital" surveyed 100 pregnant Iraqi mothers and their neonates in a cross-sectional study. Age, delivery method, gender, weight, adequate sun exposure, and time in utero were all determined for the mother. Enzyme-Linked Immunosorbent Assay is used to assess Vitamin D in both mothers and infants (ELISA). Results: Among a sample of 100 mothers and their children, researchers discovered that 95% of moms and 86% of newborns had insufficient or deficient Vitamin D. Vitamin D of parents and newborns was found to be different. Infant Vitamin D is favorably associated with maternal Vitamin-D status. Birth weight is strongly associated with maternal Vitamin D. Vitamin D in women was (5.1-19.1) ng/ml whereas in neonates was (9.28-31.22) ng/ml, on average. Conclusions: Vitamin D deficiency or insufficiency is widespread, particularly among mothers, but it is also present in a considerable percentage of babies. There is a link between the mother's Vitamin D level and that of their babies.