Lifting the corms of saffron (Crocus sativus L.) after leaf withering (late June to early July) and storing them at 25 ºC during a variable period of time before forcing flowering at 17 ºC, allowed us to program flowering at will from mid September to mid December. An earlier flowering was obtained lifting the corms before leaf withering (by the end of May) and/or heat curing the corms at 30 ºC during 20 days before the incubation at 25 ºC. On the other hand, flowering could be delayed storing the corms at low temperature. The conditions of this cold storage were critical as regards to the stage of flower initiation of the corms, the temperature and duration of storage and the composition of the atmosphere. A departure from the optimal conditions resulted in flower abortion and/or a reduction in flower size. The combination of the techniques described allowed us to extend the harvest season of saffron in the greenhouse from early September to the end of January, with a saffron spice yield per corm higher than 17 mg. Since the mean duration of the period of flowering for a batch of corms was 13 days, we could grow 11 batches in that period. With the planting density we used (457 corms m-2); 1 hectare of greenhouse yielded 855 Kg of saffron spice in a year. The period of flowering could be extended until the end of March but with a smaller spice saffron yield per corm (over 14 mg).
En la gestación, estrógeno y progesterona aumentan sustancialmente, con marcada variabilidad interindividual generada por el aporte de grasas dietarias, entre otros factores. Objetivo: evaluar el impacto de dietas con variadas relaciones n6/n3, administradas antes y durante la gestación, en las concentraciones de estradiol (E) y progesterona (P4) y su incidencia en el éxito reproductivo (ER).Ratones hembras adultas recibieron, desde 25 días previos a la gestación, dieta comercial (C, n6/n3 = 19,10) o C suplementada con: 10% de aceite de girasol (G, n6/n3 = 87,17), 10% de aceite de pescado (P, n6/n3 = 1,29) y con 5% de aceite de girasol y 3% de aceite de pescado (Equilibrada, E, n6/n3 = 5,17). El día 16,5 de gestación se sacrificaron las madres (n=8 en todos los grupos). Se registró: ER, número de cuerpos lúteos (NCL) y peso de ovarios (PO). Se cuantificó la concentración de E2 y P4 en plasma por inmunoensayo enzimático. Estadística: ANOVA, Chi-cuadrado y Kruskall-Wallis.ER sin diferencias significativas (C: 88,88%, G: 91,66%, P: 100% y E: 72,73%). El NCL tampoco exhibió diferencias significativas. El PO fue significativamente menor en las hembras tratadas (G= 0,01 ± 0,00082; P= 0,01 ± 0,00099 y E= 0,01 ± 0,00055 vs C= 0,02 ± 0,00037; p?0,05). Las concentraciones de E2 fueron mayores en P (P= 0,09 ng/ml ± 0,02 vs C= 0,05 ng/ml ± 0,01; G= 0,06 ng/ml ±0,01 y E= 0,03 ng/ml ± 0,01; p?0,01). P4 no presentó diferencias significativas. Sin embargo, se observó una tendencia a mayores valores en el grupo P (P= 2,07 ng/ml ± 0,24; G= 1,51 ng/ml ± 0,24; E= 1,39 ng/ml ± 0,18 y C= 1,43 ng/ml ± 0,2).Los cambios en las relaciones n6/n3 no afectan el ER ni la concentración de P4. El aumento de estradiol en el grupo P, excedido en n3, podría deberse a modificaciones en el estrés oxidativo y en la función mitocondrial que pueden conducir a alteraciones en la esteroidogénesis. Estudios próximos permitirán dilucidar estos aspectos.
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