Depression and anxiety are prevalent in the stroke population and can have a significant impact on the patient and their family's long-term outcomes, however the screening for these conditions does not meet best practice recommendations. To address this deficit, this study developed "Post-Stroke Mood Assessment Pathways" and embedded them into practice by utilising the PARiHS framework (for the implementation of evidence-based practice), in conjunction with staff training. The study examined the rates of mood screening, clinical interviews, and completion of interventions for stroke patients through a retrospective chart audit (n = 213) one year prior to, and one year post-implementation (n = 238) of the pathways. The data show statistically significant increased documentation around mood screening and clinical interview 95% CI [4.86, 19.88]