This study was designed to investigation the effect of oocytes collected methods (aspiration and slicing) to oocytes recovery per ovary and the quality oocytes (normal and abnormal) in local Iraqi ewes. The female genitalia was brought from Al-shulla abattoir from the animals after slaughtered, the samples was collected and transport to reproductive techniques laboratory with 1-3 hrs in thermal box containing normal saline (0.9%) supplemented with 100 IU/ml penicillin, and 1mg / ml streptomycin. In laboratory the ovaries washed in phosphate-buffered solution with antibiotics three times. Each time the oocytes collection in different method aspiration or slicing to compared in recovery rate and quality of oocyte collected after classification four group according number of cumulus layers and uniform the cytoplasm to (A + B) when contained multiple layer of cumulus cells with homogenous cytoplasm considered normal oocytes and (C+D) when absent cumulus cells or degenerated with heterogeneous cytoplasm considered abnormal oocytes in two different methods. The result of this current study showed the high significant effect (P≤0.01) in recovery rate to slicing than aspiration (4.91, 3.23) oocyte /ovary respectively.

This study was designed to investigate the effect of addition several concentrations of royal jelly to the media used in the in vitro maturation process of Iraqi sheep. The ovary was brought from the Al-Shulla slaughterhouse and transferred to the obstetric laboratory at the College of Veterinary Medicine at the University of Baghdad by a thermal insulating box containing the physiological solution with the antibiotic penicillin at a temperature of 30-38°C. In the laboratory, the ovaries were washed with a neutral buffer solution and then the oocytes were collected by two different methods slicing ovaryor follicle aspiration. The oocytes were classification into four groups depending on the layers of the cumulus cells surrounding the oocytes, as well as the homogenous of the cytoplasm. Oocytes that are completely or partially surrounded by cumulus cells and homogeneous cytoplasm considered normal oocytes but the oocytes denuded from the cumulus cells and heterogeneous cytoplasm considered abnormal oocytes was discarded. Normal oocytes were divided randomly into four groups at maturation; the first group was matured by TCM media alone and was considered as a control group.