By reacting maleic anhydride with amines, we synthesized the derivatives N-ethyl, N-(2-ethylamine), N-piperidinyl, N-phenyl, and N-phenylhydrazinyl maleamic acids. The purity of these products was initially verified by melting range and the presence of only one spot observed by thin layer chromatography. The chemical structures of the obtained N-alkyl maleamic acids were confirmed through infrared (IR) and hydrogen and carbon nuclear magnetic resonance ( 1 H and 13 C NMR) spectrometry. Due to the already proven pharmacological activity of maleimides, maleic anhydride and its N-alkyl maleamic acids were subjected to in vitro assays to observe antiviral (SA-11 rotavirus), antibacterial (Escherichia coli, Staphylococcus aureus, and Bacillus cereus), antifungal (Colletotrichum musae, Fusarium solani f. sp. phaseoli, Fusarium solani f. sp. piperis Alb., and Penicillium sp.), and antiprotozoal (Trichomonas vaginalis, Giardia lamblia, and Entamoeba histolytica) effects. To study the anti-rotavirus properties, firstly the 3-(4,5-dimethylthiazol-2-yl)-2-5-diphenyltetrazolium bromide (MTT) method was used to establish the median cytotoxicity concentration (CC 50 ) of the compounds, using MA-104 cell line. Under the experimental conditions used, cytotoxic, anti-rotavirus, antibacterial, and antifungal properties were not observed for these compounds.

Water is the main source of transmission of micro-organisms in health centers, which can cause infections that are a serious issue for public health. The goal of this study was to evaluate the microbiological quality of water used in a hospital of the microregion of Ouro Preto, Minas Gerais, Brazil. Twelve water samples were collected at different points for the detection of total and fecal coliforms, as well as other micro-organisms; in addition, antimicrobial susceptibility tests were also performed. The bacterial species Pseudomonas aeruginosa was detected in the sample from the rinse water of clean clothes collected in the laundry's clean area and it was identified by Gram stain and automated analysis using VITEK ® equipment. The strain of P. aeruginosa showed sensitivity to the antibiotics commonly used to treat infections induced by this bacterium, such as cephalosporins, fluoroquinolones, carbapenems and polymyxins. However, the results indicate the need for a greater attention to the process of cleaning of hospital clothing, a fact that is still neglected in Brazil. Keywords: Pseudomonas aeruginosa; hospital infection; Hospital Laundry Service; microbiology water RESUMOA água representa o principal meio de transmissão de micro-organismos em centros de saúde, os quais podem causar infecções que são um grave problema de saúde pública. O objetivo desse estudo foi avaliar a qualidade microbiológica da água utilizada em um hospital da microrregião de Ouro Preto, Minas Gerais, Brasil. Foram coletadas doze amostras de água em pontos distintos para testes de detecção de coliformes totais e termotolerantes, assim como de outros microrganismos; além disso, testes de sensibilidade a antimicrobianos também foram realizados. A espécie bacteriana Pseudomonas aeruginosa foi detectada na amostra correspondente à água de enxágue de roupas limpas coletada na área limpa da lavanderia e foi identificada por coloração de Gram e análise automatizada utilizando o equipamento VITEK ®. O isolado de P. aeruginosa mostrou-se sensível aos antibióticos usualmente utilizados no tratamento de

SummaryIt is important to consider the use of the epigenome as source of complementary data for genome knowledge, which is suitable for the diagnosis of schistosomiasis. Usually, a laboratory diagnosis of schistosomiasis is performed by means of 1. Egg detection in the stool or urine by microscopy remains with limited sensitivity; 2. Immunological screening, in which positivity persists after treatment, and 3. Molecular appraisals prevail over the disadvantages of the currently used methods. In this sense, molecular methodologies are being developed based on epigenetic biomarkers, aiming to improve the diagnosis of the disease and clinical treatment as early as possible to prevent the occurrence of serious liver damage.

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