A produção homogênea de porta-enxertos resistentes à pérola-da-terra (Eurhizococcus brasiliensis Hempel, Hemiptera: Margarodidae) e adaptados à região vitivinícola do Sul do País, pela técnica de micropropagação, poderá vir a atender à demanda do setor produtivo de uva e evitar as perdas causadas pela praga. O objetivo deste trabalho foi desenvolver um protocolo de micropropagação dos híbridos 1 e 2, que possuem as qualidades genéticas e de vigor desejadas para porta-enxerto, e resistência à pérola-da-terra. A partir de gemas axilares cultivadas em meio Galzy adicionado de 3µM de bezilaminopurina (BAP), foi possível induzir a multibrotação, com um número satisfatório de brotos, passível de ser incrementado por subcultivos sucessivos no mesmo meio de cultura. Os brotos obtidos neste meio e transferidos para o meio de cultura Galzy, adicionado de 8µM.10-3 de ácido naftalenoacético (ANA), enraizaram em 100%, superando a dificuldade de enraizamento que em geral tem sido a maior barreira apresentada pela espécie V. rotundifolia e seus híbridos.

Studies were conducted to determine the influence of temperature and relative humidity (RH) on germinability and viability of Mucor piriformis spores. Spores did not survive when stored at 35 °C and their survival rate decreased rapidly at 30 °C; however, spores remained viable for more than 1 year at 0°C. RH also significantly affected spore viability. Spores held at 26 °C and 100% RH no longer germinated after 35 days, while those held at 75 or 90% RH germinated for 65 days. At 20 °C, RH had little effect on spore germinability. The effect of temperature and RH on percentage spore germination also varied. At all temperatures studied, spore viability decreased more rapidly with time at 100% RH than at 75 or 90% RH. The least favorable temperature-humidity combination, 30 °C and 100% RH, decreased spore germination from 100% to less than 1 % in 14 days. ZusammenfassungEinflufi von Temperatur und relativer Luftfeuchtigkeit auf die Keimfahigkeit von Mucor piriformis Sporen Es wurden Untersuchungen durchgefiihrt, um den Einflufi von Temperatur und relativer Luftfeuchtigkeit (RH) auf die Keim-und Lebensfahigkeit von Mucor piriformis Sporen festzustellen. Die Sporen Iiberlebten eine Lagerung bei 35 °C nicht, und ihre Uberlebensrate verringerte sich sehr schnell bei 30 °C. Jedoch bei 0°C blieben die Sporen uber ein Jahr lebensfahig. Die Lebensfahigkeit der Sporen wurde auch durch die RH signifikant beeinflufit. Sporen, die bei 26 °C und 100% RH gelagert wurden, keimten nach 35 Tagen nicht mehr, wahrend Sporen, die bei 75 oder 95% RH gelagert U.S. Copyright Clearance Center Code Statement: 0031-9481/87/1 801-0003$02.50/0 4 BERND, BEAN, CONWAY smd MOLINE wurden, 65 Tage lang keimten. Bei 20 °C hatte die RH kaum Einflufi auf die Sporenkeimfahigkeit. Die Auswirkung von Temperatur und RH auf die prozentuale Sporenkeimung war auch unterschiedlich. Bei alien untersuchten Temperaturen verringerte sich die Sporenlebensfahigkeit schneller bei 100% RH als bei 75 oder 90 % RH. Bei der ungiinstigsten Temperatur-Liiftfeuchtigkeitskombination -30 °C und 100% RH -verringerte sich die Sporenkeimung von 100% zu weniger als 1 % innerhalb 14 Tagen.

Butia odorata is a palm native to southern Brazil and Uruguay, not domesticated, much appreciated for its fruits and economic potential. However, the extractivism and the diffi culty of propagation have led to the decline of natural populations. The objective of this work was to prove the possibility of induction of somatic embryogenesis in B. odorata. Mature zygotic embryos were induced in two media, MS and Y3, combined with auxin 2,4-D and picloram in fi ve concentrations (2,4-D: 0, 361.99, 452.49, 542.99 and 633.48 μM/L, picloram: 0, 50, 150, 300 and 450 μM/L). The results promising during induction with the formation of embryogenic calli and somatic embryos, however the regeneration of them was not effi cient, this may be due to the occurrence of somatic embryos fused during its development. The roots were formed, but the aerial part remained molten, not completing its development. Auxin picloram and Y3 medium provided the most adequate conditions for calogenesis, formation of embryogenic callus and somatic embryos, with concentrations of 150, 300 and 450 μM/L. This is the fi rst description of somatic embryogenesis in B. odorata that will serve as the basis for future research and adjustments of the methodology proposed here.