Background: A chemical compound is a chemical compound present in a natural source that gives it the special properties and characteristics of the plant or animal source. Snail slime is one of the traditional medicines from animals that is used by the community as a traditional medicine for healing minor wounds and toothaches. The aim of this study was to identify the active chemical compounds and antibacterial potential of snail mucus against the bacteria E. foecalis that causes periodontitis. Method: True experiment laboratory research method which is carried out by laboratory analysis of snail mucus to obtain chemical elements, the content of compounds contained in a test sample. The content of active compounds based on the Gas Chromatography-Mass Spectrometry (GCMS) test. Results: The average content of heparan sulfate with four repetitions was 16.45 mg/100g, Acharan sulfate 21.33 mg/100g, protein achasin 102.22 mg/100g, Glycoconyugat 88.6 mg/100g, Ion Ca2+ 86.2 mg/100g, Beta agglutinins 85.22 mg/100g. Toxicity test was carried out with four repetitions with concentrations of 100%, 50%, 25%, and 12.5%. And the lowest results of the toxicity test were at a concentration of 100% with a value of 0.171, while at a concentration of 50% 0.302, 25% 0.453, and 12.5% 0.768, for bacteria E. Foecalis with an inhibitory power of 23.15 mm, the category was very strong. Conclusion: The active chemical content in snail mucus is Acharan sulfate, achasin protein, Glycoconyugat, Ca2+ ion, Beta agglutinin, and the antibacterial potential of snail mucus against E. foecalis bacteria is very strong.

Background: Kirinyuh (Chromolaena odorata L.) leaf as a traditional medicine is used for wound healing. Kirinyuh is easy to get because it can still grow well in less fertile areas. This study aims to determine the results of qualitative and quantitative tests of the active substance in kirinyuh leaf extract (Chromolaena odorata L) growing in the province of Bali.Methods: This research was pre-experimental with completely randomized design with post-test only control group design. The research sample is kirinyuh leaves from three regions in the province of Bali based on geomorphology. Qualitative test results of ethanol and water extract of kirinyuh leaves (Chromolaena Odorata L.) methanol and ethyl acetate fractions contain alkaloids, saponins, tannins, phenols, flavonoids and antioxidants.Results: The results of the quantitative test of the highest alkaloid content from the ethanol extract of the methanol fraction from Jimbaran, namely 11,690.49 mg/100 gr. The highest tannin content of the ethanol extract of the ethyl acetate fraction from Tabanan was 172777 mg/100 gr. The highest Saponin content from the aqua extract of the ethyl acetate fraction was 2977.27 mg/100gr. The highest phenol content from the ethanol extract of the methanol fraction from Bangli was 19716.1 mg/100 gr. The highest flavonoid content from the ethanol extract of the ethyl acetate fraction from Tabanan was 13704.7 mg/100 gr. The highest antioxidant content of the aqua extract of the methanol fraction from Tabanan was 62.3067 ppm.Conclusion: Total alkaloids of kirinyuh leaves in Jimbaran, Bangli and Tabanan were significantly different with p value = 0.000 (p < 0.05), tannins with p value = 0.000, phenols with p value = 0.006, flavonoids with p value = 0.000 and antioxidants with p value. p=0.000. Saponins in extracts from Jimbaran, Bangli and Tabanan there was no significant difference because the value of p=0,252 (p>0,05). Latar Belakang: Daun kirinyuh ( Chromolaena odorata L. ) sebagai obat tradisional digunakan untuk penyembuhan luka. Kirinyuh  mudah didapat karena dapat tetap tumbuh baik di daerah yang kurang subur. Penelitian ini bertujuan untuk mengetahui hasil uji kualitatif dan kuntitatif zat aktif dalam ekstrak daun kirinyuh (Chromolaena odorata L) yang tumbuh di propinsi Bali.Metode: penelitian ini adalah pra eksperimen dengan rancangan completely randomized with post test only control group design. Sampel penelitian yaitu daun kirinyuh dari tiga wilayah di provinsi Bali berdasarkan geomorfologi. Hasil uji kualitatif  ekstrak etanol dan air daun kirinyuh (Chromolaena Odorata L.) fraksi methanol dan etil asetat mengandung senyawa alkaloid, saponin, tannin, fenol, flavonoid dan antioksidan.Hasil: Hasil uji kuantitatif kadar alkaloid terbanyak dari ekstrak etanol fraksi methanol dari Jimbaran, yaitu 11.690,49 mg/100 gr. Kadar tannin terbanyak dari ekstrak etanol fraksi etil asetat dari Tabanan, yaitu 172777 mg/100 gr.  Kadar Saponin terbanyak dari ekstrak aqua fraksi etil asetat yaitu 2977,27 mg/100gr. Kadar fenol terbanyak dari ekstrak etanol fraksi methanol dari Bangli, yaitu 19716,1 mg/100 gr. Kadar flavonoid terbanyak dari ekstrak etanol fraksi etil asetat dari Tabanan, yaitu 13704,7 mg/100 gr.  Kadar antioksidan terbanyak dari ekstrak aqua fraksi metanol dari Tabanan, yaitu 62,3067 ppm.Simpulan: Total alkaloid daun kirinyuh Jimbaran, Bangli dan Tabanan berbeda bermakna dengan nilai p=0,000 (p<0,05), tannin dengan nilai p=0,000, fenol dengan nilai p=0,006, flavonoid dengan nilai p=0,000 dan antioksidan dengan nilai p=0,000.  Saponin dalam ekstrak dari Jimbaran, Bangli dan Tabanan tidak ada perbedaan bermakna karena nilai p=0,252 (p>0,05).

Kirinyuh leaves (Chromolaena odorata L.) are natural medicinal plants for wound healing. WHO (World Health Organization) recommends and concern about the safety of plant medicines. Due to limited toxicity study of Chromolaena odorata, this study aimed to determine the acute toxicity of the ethanolic extract of C. odorata in terms of LD50 and its effect on liver and kidney function of male white rats. The observed toxicity parameters were LD50 and delayed toxic effects for 14 days, including toxic symptoms, body weight, AST, ALT and creatinine test, liver and kidney histopathology. The study used 24 white male rats divided into 4 groups, namely the control group NaCMC 0.5%, the treatment group of C. odorata leaf ethanol extract 5 g/kg bw, 10 g/kg bw and 15g/kg bw with a single dose. The results showed that no white rats died during the 14 days of observation. Toxic symptoms that appear only white rats lack of appetite. Other parameter was body weight between days 1, 3, 7, 10 and 14 for each treatment group was significantly different and tended to decrease. The AST, ALT and creatinine values ??were not significantly different for all treatments. Histopathology of the liver and kidneys showed no abnormalities. Based on these results, the LD50 of kirinyuh leaf extract was 15 g/kg bw and delayed toxic effects in the form of lack of appetite which caused weight loss in all treatment groups.