Aspirin is one of the most commonly used drugs. It is necessary to find and develop analgesic anti- inflammatory drugs that are safe against their adverse effects. From this standpoint, new nano- organometallic Cu(II), Mn(II), Ni(II), Cd(II) and Zn(II) compounds of modified aspirin were prepared and characterized using elemental and spectroscopic techniques such as infrared and ultraviolet, proton nuclear magnetic resonance, mass spectroscopy, electron spin resonance and electron microscope, as well as magnetism, conductivity and thermal analysis. Thermal and spectral results showed the octahedral geometry and stability of these compounds up to 45oC. The data indicated that, using low concentration against microbes, aspirin and ligand no effect was observed, however, the complexes showed moderate to high effect, The order for Gram positive is Cd(II) complex (10)> Cd(II) complex (11)> ampicillin> Zn(II) complex (8)> Cu(II) complex (4)> Cu(II) complex (6)= Ni(II) complex (12)> Cu(II) complex (2) = Cu(II) complex (3)> ligand (1)> aspirin> Mn(II)complex(14) and for Gram negative is ampicillin> Cd(II) complex (10)> Cd(II) complex (11)> Cu(II) complex (2) > Cu(II) complex (6)> Cu(II) complex (3)> Cu(II) complex (4) > Zn(II) complex (8)> aspirin> Ni(II) complex (12) > ligand (1)> Mn(II) complex (14) and for Aspergillus flavus is Cd(II) complex (10)>amphotericin B> Cd(II) complex (11)> ligand (1)= aspirin= Cu(II) complex (2)= Cu(II) complex (3)= Cu(II) complex (4)= Cu(II) complex (6) = Zn(II) complex (8)= Ni(II) complex (12)= Mn(II) complex (14) and for Candida albicans is Cd(II) complex (10)> Cd(II) complex (11)> amphotericin B > Ni(II) complex (12)> Cu(II) complex (3)= Cu(II) complex (6)> Cu(II) complex (2)= Cu(II) complex (4)> ligand(1) = aspirin= Zn(II) complex (8)= Mn(II) complex (14). The order of the catalytic effect of the tested complexes on decomposition of H2O2 was Mn(II) complex (14)> Ni(II) complex (12) > Cu(II) complex (4) > Cu(II) complex (6)> Cu(II) complex (2) respectively.