A wild fruit from classified forest of Dindéresso was analyzed for total phenolics, flavonoids, anthocyanins compounds using the Folin-Ciocalteu reagent, spectrophotometric method of Zhishen and colleagues and by the differential pH method respectively. Free radical-scavenging activities of studied fruits extracts were estimated using diammonium 2,2'-azino-bis-(3-ethylbenzothiazoline-6-sulfonate) salt method. Three major anthocyanins were identified using high performance liquid chromatography coupled with spray ionization interface mass spectrometry. Three identified anthocyanins in fruit were reported to be cyanidin 3-O-(p-coumaroyl) glucoside, pelargonidin 3-O-glucoside and pelargonidin 3-O-rutinoside. In addition, H. monopetalus fruit contained of about 1165±3.1 mg of GAE per 100 g of fresh fruit, 4490±20.2 mg of QE per 100 g of fresh fruit, and 36±0.17 mg of cyanidin 3-O-glucoside equivalents per 100 g of fresh fruit. Total anthocyanin extract had an EC50 = 4.24 mg per mL and a TEC50 time of 21 minutes (intermediate reaction). This free radical-scavenging activity was very low compared to those of the references used (0.024 and 0.034 mg/mL respectively for ascorbic acid and Trolox). The low antiradical activity and reactivity of the H. mucronata extract could be explained by several factors. In any case, fruits of this species were potential sources of natural bioactive substances having beneficial effects on the health of consumers.

the most important and harmful tick species in the livestock sector in Burkina Faso today. This tick has developed resistance to most classes of synthetic acaricides. The objective of this work is to assess the efficiency of plant extracts on this tick to find an alternative. Thus, the acaricidal activity of hydroethanolic and hexanic extracts of Ocimum americanum .L, Ocimum gratissimum .L and Laggera oloptera (DC) Adams was evaluated on adults of this tick by the adult immersion method. The mortality, the inhibition of the laying, the hatching rate, and the effectiveness of each extract have been evaluated with concentrations at 100 mg/mL and at 200 mg/mL, respectively. The best mortality rate was 56%, with the hydroethanolic extract of L. oloptera at 200 mg / mL. The hydroethanolic extract of O. gratissimum was most effective with the best rates of egg inhibition and hatching rates at 200 mg / mL. The results of this study could be used for the development of new bio-acaricide formulations to fight against the tick R. (Boophilus) microplus effectively.Keywords: Ticks, O. gratissimum, O. americanum, Laggera oloptera, phytochemical screening

Les huiles essentielles constituent une source potentielle de molécules à activité antioxydante. Le développement des combinaisons optimisées de ces substances naturelles permettrait leur utilisation comme conservateurs naturels. Ainsi, l’objectif de la présente étude est de déterminer la composition chimique de l’huile essentielle obtenue par co-distillation de Mentha piperita L. et de Cymbopogon citratus DC. Stapf du Burkina Faso et de comparer son potentiel antiradicalaire à celui du mélange obtenu par combinaison des huiles essentielles pures de ces deux plantes. Les huiles essentielles ont été extraites à l’aide d’un appareil de type Clevenger par hydrodistillation des feuilles sèches de Cymbopogon citratus et Mentha piperita, séparément, puis à partir du mélange de leurs feuilles sèches dans les proportions massiques 80% pour C. citratus et 20% pour M. piperita. Elles ont été ensuite analysées par chromatographie en phase gazeuse couplée à la spectrométrie de masse. Le potentiel antiradicalaire des huiles essentielles, exprimé en milligramme d’équivalent d’acide ascorbique par millilitre d’huile essentielle (mg EAA/mL), a été évalué par la méthode de DPPH. L’huile essentielle obtenue par co-distillation contient majoritairement le citral (49,26%), le β-myrcène (10,98%), le menthol (5,90%) et la menthone (4,50%). L’essence de C. citratus est dominée par le citral (73,77%) et le β-myrcène (13,66%) et celle de M. piperita par le menthol (31,54%), la menthone (20,27%), le menthofurane (15,10%) et l’acétate de menthyle (8,59%). Le pouvoir antiradicalaire des huiles essentielles est : 11,73 ± 0,14 mg EAA/mL pour l’essence de M. piperita ; 10,01 ± 0,06 mg EAA/mL pour celle de C. citratus ; 40,13 ± 0,08 mg EAA/mL pour l’essence obtenue par co-distillation et 11,96 ± 0,04 mg EAA/mL pour l’essence obtenue par combinaison des essences pures. Ces résultats montrent que la co-distillation a permis d’améliorer le potentiel antiradicalaire des huiles essentielles des deux plantes et ouvre des perspectives pour son utilisation comme antioxydant naturel. Essential oils are a potential source of molecules with antioxidant activity. The development of optimized combinations of these natural substances should allow their use as natural preservatives. Thus, the objective of this study is to determine the chemical composition of essential oil obtained by co-distillation of Mentha piperita and Cymbopogon citratus from Burkina Faso and to compare its antiradical potential with that of the mixture obtained by combining pure essential oils of these two plants. Essential oils were extracted using a Clevenger-type apparatus by hydrodistillation of dry leaves of Cymbopogon citratus and Mentha piperita, separately, then from the mixture of their dry leaves in the mass ratio 80% for C. citratus and 20% for M. piperita. They were then analyzed by gas chromatography / mass spectrometry. The antiradical potential of essential oils expressed in milligrams of ascorbic acid equivalent per milliliter of essential oil (mg EAA/mL) was evaluated by DPPH method. Essential oil obtained by co-distillation mainly contains citral (49.26%), β-myrcene (10.98%), menthol (5.90%) and menthone (4.50%). Essential oil of C. citratus is dominated by citral (73.77%) and β-myrcene (13.66%) and that of M. piperita by menthol (31.54%), menthone (20.27%), menthofuran (15.10%) and menthyl acetate (8.59%). Antiradical potential of essential oils are : 11.73 ± 0.14 mg EAA/mL for the essential oil of M. piperita ; 10.01 ± 0.06 mg EAA/mL for that of C. citratus ; 40.13 ± 0.08 mg EAA/mL for essential oil obtained by co-distillation and 11.96 ± 0.04 mg EAA/mL for essence obtained by combining pure essential oils. These results show that co-distillation has improved antioxidant efficiency of the essential oils of these two plants and opens prospects for its use as a natural antioxidant.

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