RESUMO: "Estudos de toxicidade aguda e crônica do produto fi toterápico brasileiro Ierobina ® ". A Ierobina ® é um produto utilizado popularmente, no Brasil, para tratamento de dispepsia, na dose de 280 mg/kg/dia. Apesar de seu largo uso nos últimos 75 anos, recentemente foi comprovada sua efi cácia em animais; porém, nenhuma avaliação de seu perfi l toxicológico foi realizada. O objetivo do presente estudo foi avaliar a toxicidade aguda (doses únicas de 2100 mg/kg, 6300 mg/kg ou 12600 mg/kg), em camundongos, e crônica (doses de 2800 mg/kg ou 5600 mg/kg, por 180 dias), em ratos, após administração per os de Ierobina ® . No teste de toxicidade aguda, as doses administradas não produziram nenhuma mortalidade e os sinais observados foram todos reversíveis. No teste de toxicidade crônica, não foram verifi cadas diferenças signifi cativas nas análises hematológicas, macroscópicas e microscópicas. Nos exames de bioquímica sérica, diferença signifi cativa foi observada somente na avaliação da alanina transaminase, aspartato transaminase e creatinina, porém, sem importância clínica. Assim, considerando os resultados obtidos e o fato de ser a Ierobina ® um produto comercializado há décadas, sem qualquer notifi cação de casos de toxicidade, podemos concluir que o produto parece ser de segurança adequada para uso humano. Unitermos: Ierobina® , toxicidade aguda e crônica, produto fi tofarmacêutico.ABSTRACT: Ierobina ® is a Brazilian phytopharmaceutical product employed for the treatment of dyspepsia (280 mg/kg/day). Despite its widespread use in the country for over 75 years, only recently its therapeutic effi cacy has been attested in animals; however, no toxicological investigations have been carried out for the product to date. In this paper we evaluated the acute toxicity of Ierobina ® administrated by gavage in mice (single doses of 2100 mg/kg, 6300 mg/ kg and 12600 mg/kg), along with its chronic effects in rats, after product administration per os daily, at the doses of 2800 mg/kg and 5600 mg/kg, for 180 days. The product had low acute toxicity; all observed alterations were reversible and no animal died during the experiments. In chronic toxicological studies, Ierobina ® administration for 180 days did not cause any changes in hematological and biochemical parameters, with the exception of decreasing the levels of alanine transaminase, aspartate transaminase and creatinine. However, histological evaluation of kidney, liver and other selected organs showed normal architecture, suggesting no morphological disturbances. Hence, considering the obtained results and the fact that Ierobina ® has been commercialized for decades in Brazil, without any notifi ed case of toxicity, it seems that the product is safe for human use.

The burning of organic residues and wastes in furnaces of cement industries has been an attractive and lucrative approach to eliminate stocks of these pollutants. There is a potential risk for producing PAH in the workplace of industries burning organic wastes, so that highly sensitive analytical methods are needed for monitoring the air quality of these environments. An official method for determination of PAH is based on liquid chromatography with fluorescence detection at fixed excitation and emission wavelengths. We demonstrate that a suitable choice of these wavelengths, which are changed during the chromatographic run, significantly improves the detectability of PAH in atmosphere and particulate matter collected in cement industries.