Yeasts are common inhabitants of the phyllosphere, but our knowledge of their diversity in various plant organs is still limited. This study focused on the diversity of yeasts and yeast-like organisms associated with matured fruits and fully open blossoms of apple, plum, and pear trees, during 2 consecutive years at 3 localities in southwest Slovakia. The occurrence of yeasts and yeast-like organisms in fruit samples was 2½ times higher and the yeast community more diverse than that in blossom samples. Only 2 species (Aureobasidium pullulans and Metschnikowia pulcherrima) occurred regularly in the blossom samples, whereas Galactomyces candidus, Hanseniaspora guilliermondii, Hanseniaspora uvarum, M. pulcherrima, Pichia kluyveri, Pichia kudriavzevii, and Saccharomyces cerevisiae were the most frequently isolated species from the fruit samples. The ratio of the number of samples where only individual species were present to the number of samples where 2 or more species were found (consortium) was counted. The occurrence of individual species in comparison with consortia was much higher in blossom samples than in fruit samples. In the latter, consortia predominated. Aureobasidium pullulans, M. pulcherrima, and S. cerevisiae, isolated from both the fruits and blossoms, can be considered as resident yeast species of various fruit tree species cultivated in southwest Slovakia localities.

The tolerance of seventy yeast strains belonging to 15 species, isolated from water and soil environments as well as from tree leaves, to four heavy metals--copper, zinc, nickel and cadmium were studied. We have found that the interspecific and intraspecific variations in metal tolerance among studied strains were considerable. The highest interspecific variations were observed toward copper and cadmium. The strains of the species Sporobolomyces salmonicolor, Cryptococcus albidus, Cystofilobasidium capitatum, Saccharomyces cerevisiae, and Candida maltosa belonged to the most sensitive ones. In general ascomycetous yeasts were more tolerant to heavy metals than basidiomycetous ones. The differences among strains that came from various natural sources were also found. The most sensitive yeast population originated from untilled soil whereas the most tolerant population was isolated from tree leaves.

One hundred and eleven yeast strains were isolated from 60 agricultural soil samples. The samples were taken from four various fields located in the southwest of Slovakia. Cryptococcus laurentii, Candida maltosa, Metschnikowia pulcherrima, and Sporobolomyces salmonicolor were the predominant species in the samples collected from all four types of fields. These species represented 78.4-86.6% of the total yeast counts.The results obtained enabled comparisons to be made between forest and agricultural soil yeast population. We have found out that the yeast population in tilled soils was significantly reduced. The number of yeasts in the tilled soils ranged from 40 to 6.8 x 10(3) CFU/g soil and the average number reached approximately 1.12 x 10(3). This number is more than ten times lower in comparison with the forest soils.