The supply of petroleum-based fuel decreases, and the need to produce viable renewable fuel increases. In the wake of the uprising global energy crisis, microalgae serve as a promising feedstock for oil production. The researcher gained interest due to its hitting-two birds-with-one-stone approach as a potential solution for the continuously depleting source of oil reservoir worldwide and to reduce carbon dioxide emission into the atmosphere using microalgae. The potential of the strains (Chlorella vulgaris, Monoraphidium sp., Scenedesmus obliquus, Desmodesmus denticulatus, and Chlorophyta sp.) in the production of lipid was assessed in this study. Furthermore, optimization of the growth conditions of each microalgae strain were identified to enhance biomass production and lipid accumulation. Two microalgae strains namely Monoraphidium sp. and Desmodesmus denticulatus exhibited higher lipid productivity compared to other microalgae strains on biomass and lipid content. The following optimized growth conditions that support algal growth are as follows: pH 5, 0.5 moles in salinity level, outside temperature, and sunlight. The production of lipids is dependent on the microalgae strain, including the optimum condition that supports its biomass production.