RESUMO:Este trabalho teve como objetivo utilizar métodos de controle de qualidade para algumas drogas vegetais a base de Bauhinia forfi cata comercializadas nos municípios de Itajaí e Balneário Camboriú, utilizando como marcador químico o fl avonóide kaempferitrina. As metodologias empregadas foram cromatografi a em camada delgada (CCD) e cromatografi a líquida de alta efi ciência (CLAE), além da análise microscópica, análise de rótulos e características organolépticas e a presença de material estranho. Todas as análises foram comparadas com os resultados demonstrados pela amostra autêntica, devidamente identifi cada, de B. forfi cata (A). Foram analisadas 06 amostras a base de Bauhinia identifi cadas através de siglas, sendo a B. forfi cata autêntica a amostra A e seis comerciais denominadas B-G. Todas as amostras comerciais foram reprovadas quanto à presença de material estranho, fi cando acima de 2%. Na análise microscópica observou-se que apenas a amostra E e G apresentam-se iguais à B. forfi cata. A análise cromatográfi ca revelou a presença de kaempferitrina somente nas amostras B, C, E e G. Unitermos: Bauhinia forfi cata, controle de qualidade, kaempferitrina.ABSTRACT: "Quality control of drugs with Bauhinia forfi cata Link (Fabaceae)". The present study aimed to use methods to perform the quality control of some vegetal drugs based on Bauhinia forfi cata from Itajaí and Balneário Camboriú, using the fl avonoid kaempferitrin as chemical marker. The methods used were thin layer chromatography (TLC) and high performance liquid chromatography (HPLC), besides microscopic analysis, label analysis and organoleptic characteristics and strange material presence. All the analyses were compared with the authentic sample of B. forfi cata (A) results. This study used six commercial samples which were denoted as B-G. In the strange material analyses, all the samples were disapproved because they were above 2%. Microscopic analyses revealed that only B and G samples were identical to B. forfi cata (A). The chromatographic results suggested that only the samples B, C, E and G presented kaempferitrin.

RESUMO:O presente trabalho teve por objetivo o estabelecimento de parâmetros para análise morfo-anatômica das partes aéreas (caule e folhas) da planta Wedelia paludosa DC., Asteraceae. A droga vegetal e o respectivo pó foram submetidos às análises macro e microscópica após preparação de lâminas semi-permanentes de cortes transversais e paradérmicos. O pó foi analisado após diafanização com cloral hidratado. É uma planta herbácea com caule castanho-avermelhado, folhas opostas, curto-pecioladas e membranáceas, apresentando pêlos nas duas faces, estreitada em direção à base acima do meio provida de dois pequenos lobos laterais e um terminal, maior e denteado. A planta apresenta secção circular de caule, que em crescimento primário, possui epiderme, colênquima angular, parênquima cortical e cilindro vascular, assim como oxalato de cálcio (várias drusas e alguns cristais prismáticos). A folha é simples, contendo tricomas tectores e estômato anomocítico. O mesofilo possui organização dorso-ventral e a nervura central, em corte transversal, apresenta-se côncavo-convexo com três feixes colaterais e presença de drusas de oxalato de cálcio nas células parenquimáticas. No pó obtido da droga vegetal foram verificadas as mesmas estruturas microscópicas observadas na droga vegetal rasurada. Os parâmetros morfoanatômicos estabelecidos neste trabalho permitem o controle de qualidade macro e microscópico da planta tanto rasurada como na forma de pó.Unitermos: Acmela brasiliensis, controle de qualidade, plantas medicinais, Sphagneticola trilobata, Wedelia paludosa, Asteraceae.ABSTRACT: "Morphoanatomic analysis of aerial parts of Wedelia paludosa DC. (Acmela brasiliensis, Sphagneticola trilobata), Asteraceae". This study aimed to establish parameters for morphoanatomical analysis of the aerial parts (stem and leaves) of the plant Wedelia paludosa DC., Asteraceae. The drug and its respective powder were characterized by macro and microscopic analysis after obtaining semi-permanent lamina of transverse and paradermal sections. The powder was analyzed after being cleared with chloral hydrate. It is an herbaceous plant with reddishbrown stems, opposite leaves, short-petiolated and membranous, with hair on both faces, narrowed towards the base up the middle provided with two small lateral lobes and a, large and jagged terminal. The plant has circular cross-section of stem, in which the primary growth, has epidermis, angular collenchyma, cortical parenchyma and vascular cylinder, as well as calcium oxalate (several clusters and some prismatic crystals). The leaf is simple, containing trichomes and stomata. The mesophyll has dorsiventral organization and the midrib, in cross section presents concave-convex face with three bundles and presence of calcium oxalate in parenchymal cells. In the powder was found the same structures observed in microscopic drug erased. The morphoanatomical parameters established in this work allow the macro and microscopic quality control of power and sectioned drug.

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The triterpenes friedelin (1), β-friedelinol (2) and 3,15-dioxo-21α-hydroxyfriedelane (3) in the aerial parts of Maytenus robusta, a Brazilian medicinal plant with antiulcer potential, were seasonally quantified by gas chromatography flame-ionization detection (GC-FID) using an external standard. The method was found to be linear, precise and sensitive. Compounds 1 and 2 were found in M. robusta leaves and branches, with highest concentrations in the leaves collected in autumn, i.e. 3.21 ± 0.16 and 12.60 ± 1.49 mg g-1 dry weight of 1 and 2, respectively. On the other hand, compound 3 was found only in the branches, with the highest concentrations in winter and autumn (0.21 ± 0.01 and 0.20 ± 0.02 mg g-1). The results allow to define the optimal season and plant parts for the collection of M. robusta as a phytotherapeutic drug.

The aim of this study was to describe, for the first time, the microscopic characteristics of Piper mosenii C. DC. (Piperaceae) leaves and the phytochemical composition of the aerial parts of the species grown wild in Brazil. Macroscopic analysis of the leaves was made with naked eye and supplemented with stereomicroscopy. The leaves showed similar venation than observed for other Piper species, but with a greater amount of colenchyma cells. The absence of endoderm was observed in the cross section of the leaf, which is a characteristic not reported for other Piper species. The phytochemical investigation resulted in the isolation of four benzoic acid derivatives (1-4), two chromanones (5, 6) and a dihydrochalcone (7). Compound 6, 2,2-dimethyl-6-carboxychroman-4-one acid is being described for the first time in Piperaceae, and the compound (4) 3-(1'-oxo-3'-methyl-2'-butenyl)-4-methoxy-benzoic acid is being reported for the first time in the literature.